Quantifying RNA allelic ratios by microfluidic multiplex PCR and sequencing

被引：69

作者：

Zhang, Rui ^{[1
]}

Li, Xin ^{[2
]}

Ramaswami, Gokul ^{[1
]}

Smith, Kevin S. ^{[2
]}

Turecki, Gustavo ^{[3
]}

Montgomery, Stephen B. ^{[1
,2
]}

Li, Jin Billy ^{[1
]}

机构：

[1] Stanford Univ, Dept Genet, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Pathol, Stanford, CA 94305 USA

[3] McGill Univ, Douglas Mental Hlth Univ Inst, McGill Grp Suicide Studies, Montreal, PQ, Canada

来源：

NATURE METHODS | 2014年 / 11卷 / 01期

基金：

美国国家卫生研究院;

关键词：

EDITING SITES; REGULATORY VARIATION; GENE-EXPRESSION; IDENTIFICATION; GENOME; SEQ; TRANSCRIPTS; EVOLUTION; SELECTION; VARIANTS;

D O I：

10.1038/nmeth.2736

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a targeted RNA sequencing method that couples microfluidics-based multiplex PCR and deep sequencing (mmPCR-seq) to uniformly and simultaneously amplify up to 960 loci in 48 samples independently of their gene expression levels and to accurately and cost-effectively measure allelic ratios even for low-quantity or low-quality RNA samples. We applied mmPCR-seq to RNA editing and allele-specific expression studies. mmPCR-seq complements RNA-seq for studying allelic variations in the transcriptome.

引用

收藏

页码：51 / +

页数：6

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