Microchip flow cytometry using electrokinetic focusing

被引：188

作者：

Schrum, DP ^{[1
]}

Culbertson, CT ^{[1
]}

Jacobson, SC ^{[1
]}

Ramsey, JM ^{[1
]}

机构：

[1] Oak Ridge Natl Lab, Oak Ridge, TN 37831 USA

来源：

ANALYTICAL CHEMISTRY | 1999年 / 71卷 / 19期

关键词：

D O I：

10.1021/ac990372u

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Flow cytometry of fluorescently labeled and unlabeled latex particles is demonstrated on a microfabricated device. The latex particles were detected and counted using laser light scattering and fluorescence coincidence measurements. Sample confinement was accomplished using electrokinetic focusing at a cross intersection, and detection occurred 50 mu m downstream from the intersection. Particles with diameters of 1 and 2 mu m were analyzed and distinguished from each other based on their light scattering intensity and fluorescence. A maximum sample throughput of 34 particles/s was achieved, Sample mixtures with varying proportions of fluorescently labeled and unlabeled particles were also analyzed and found to be within experimental error of the expected ratios.

引用

页码：4173 / 4177

页数：5

共 29 条

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