Integrated capillary electrophoresis devices with an efficient postcolumn reactor in planar quartz and glass chips

被引:180
作者
Fluri, K
Fitzpatrick, G
Chiem, N
Harrison, DJ
机构
[1] UNIV ALBERTA,DEPT CHEM,EDMONTON,AB T6G 2G2,CANADA
[2] ALBERTA MICROELECT CTR,EDMONTON,AB T6G 2T9,CANADA
关键词
D O I
10.1021/ac9604090
中图分类号
O65 [分析化学];
学科分类号
070302 [分析化学]; 081704 [应用化学];
摘要
Methods to fabricate planar capillary electrophoresis devices integrated with a postcolumn reactor in fused silica (guard) and Pyrex glass are presented. Quartz is etched at similar to 1 mu m/min with a 2.1:1 width-to-depth ratio using a Cr/Au/Cr metal mask and concentrated HF/HNO3. On-chip postcolumn reaction of o-phthaldialdehyde (OPA) and amino acids gave theoretical plate numbers up to 83 000 and similar to 90 ms peak widths, corresponding to 14 plates/V and a 0.5 mu m theoretical plate height. The reactor geometry caused only a 10% degradation in efficiency.
引用
收藏
页码:4285 / 4290
页数:6
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