An atypical RNA polymerase involved in RNA silencing shares small subunits with RNA polymerase II

被引：100

作者：

Huang, Linfeng ^{[1
,3
]}

Jones, Alexandra M. E. ^{[1
]}

Searle, Iain ^{[3
]}

Patel, Kanu ^{[3
]}

Vogler, Hannes ^{[3
]}

Hubner, Nina C. ^{[2
]}

Baulcombe, David C. ^{[1
,3
]}

机构：

[1] John Innes Ctr, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

[2] Max Planck Inst Biochem, Dept Prote & Signal Transduct, D-82152 Martinsried, Germany

[3] Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2009年 / 16卷 / 01期

关键词：

DIRECTED DNA-METHYLATION; IV; TRANSCRIPTION; ARABIDOPSIS; YEAST; SIRNA; RPB7; FORM;

D O I：

10.1038/nsmb.1539

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genetic evidence indicates that plant-specific homologs of DNA-dependent RNA polymerase (Pol) II large subunits form Pol IV and Pol V complexes involved in small interfering RNA production and RNA-directed DNA methylation. Here we describe evidence that Pol V contains subunits shared with Pol II, but that RNA polymerase II subunit (RPB)-4 is missing from Pol V and that RPB5 is present as a Pol V-specific isomer, RPB5b. Pol V also has other proteins that are not present in Pol II, consistent with a role of this complex as an effector of silencing.

引用

页码：91 / 93

页数：3

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