A rapid and sensitive enzyme linked immunofilter assay (ELIFA) for whole bacterial cells

被引：17

作者：

Paffard, SM

Miles, RJ

Clark, CR

Price, RG

机构：

[1] UNIV LONDON KINGS COLL,DIV LIFE SCI,LONDON W8 7AH,ENGLAND

[2] PIERCE CHEM CO,ROCKFORD,IL 61107

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1996年 / 192卷 / 1-2期

关键词：

immunodetection; immunofiltration; Escherichia coli;

D O I：

10.1016/0022-1759(96)00029-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved method is described for the detection of Escherichia coli by an enzyme linked immunofilter assay (ELIFA) using introcellulose membrane sandwiched between two 96-well plates. The incorporation of a pumping system permits a continuous flow of reagents and/or wash fluids through the membrane and provides an assay procedure capable of detecting 10(3) bacteria per well within 40 min. Quantitative bacterial detection was based on precipitated chromogen determined by scanning densitometry. The procedure represents a significant improvement in assay time and/or sensitivity over previously described ELIFA and ELISA methods for whole bacterial cells.

引用

页码：133 / 136

页数：4

共 6 条

[1] A RAPID, SENSITIVE METHOD FOR DETECTION OF ALKALINE-PHOSPHATASE CONJUGATED ANTI-ANTIBODY ON WESTERN BLOTS [J].