Polyglycolic acid filaments guide Schwann cell migration in vitro and in vivo

被引：28

作者：

Hu, Wen ^{[1
]}

Gu, Jianhui ^{[2
]}

Deng, Aidong ^{[2
]}

Gu, Xiaosong ^{[1
]}

机构：

[1] Nantong Univ, Jiangsu Key Lab Neuroregenerat, Nantong 226001, Jiangsu, Peoples R China

[2] Nantong Univ, Affiliated Hosp, Dept Hand Surg, Nantong 226001, Jiangsu, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 11期

基金：

中国国家自然科学基金;

关键词：

cell migration; nerves; polyglycolic acid; rats; Schwann cells;

D O I：

10.1007/s10529-008-9795-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Nerve conduits filled with longitudinal aligned filaments have demonstrated a better regenerative outcome for bridging large peripheral nerve gaps than hollow nerve conduits. In the present study, we investigated the in vitro and in vitro cellular behavior of Schwann cells on polyglycolic acid (PGA) filaments by immunocyto/histochemistry and light/electron microscopy. After 1-3-week culture of rat dorsal root ganglia (DRGs) onto PGA filaments, Schwann cells from rat DRGs adhered to and migrated along PGA filaments. Twenty-four rats received implantation of chitosan conduits inserted with PGA filaments to bridge 10-mm-long sciatic nerve gaps. At 1, 2, 3 and 4 weeks post-implantation (n = 6, each time point), Schwann cells were found to migrate along PGA filaments and form cell columns resembling bands of Bungner. These results suggest that PGA filaments may play a contact guidance role in Schwann cell migration and thus serve as a promising conduit-filling material to facilitate peripheral nerve repair.

引用

页码：1937 / 1942

页数：6

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