Energy storage and optical cross-section of PS I in the cyanobacterium Synechococcus PCC 7002 and a psaE- mutant

In vivo electron flow in the unicellular cyanobacterium Synechococcus sp. PCC 7002 was studied by pulsed, time-resolved photoacoustics (PTRPA). Using 1-mu s, 2 mu J or 10(13) h nu cm(-2) pulses at 695 nm, we observed large (42 +/- 2%) Photosystem I (PS I) cyclic energy storage (ES) in the period of 2 to 12 ms after excitation with wild type (WT) intact cells. This cyclic ES was insensitive to flash interval from 0.3 to 10 s and to the presence of 1 mu m DCMU (3-(3,4-dichlorophenyl)-1,1-dimethylurea). At this low flash energy and in the absence of continuous background light (in the dark), antimycin A, carbonylcyanide-m-chlorophenylhydrazone (CCCP), 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB), DCMU, 2-n-heptyl-4-hydroxyquinoline-N-oxide (HQNO), myxothiazol and N-ethylmaleimide (NEM) caused little or no inhibition of PS I cyclic electron flow. When continuous background far-red light (lambda > 715 nm) was added during the measurement, strong inhibition by DBMIB and NEM and less by HQNO was observed, the amplitude of which was related to both concentration and the intensity of the background Light. Analysis of the data with DBMIB yields its binding constant, 1 mu m, and the turnover time of the system (> 20 ms). A turnover time of the uninhibited system of 2-3 ms was obtained by a pump-probe method. A dramatic lifting of the partial inhibition in the presence of far-red Light was caused by antimycin A and a smaller effect by myxothiazol. The rescuing effect was assigned to a 'short circuiting' of the electron flow about the cytochrome (cyt) b(6)/f system. Progressively increasing the laser pulse energy allowed us to calculate the PS I optical cross-section (54 +/- 2 Angstrom). Analysis by the sensitive method of convolutions revealed a possible energy loss on the few ms time scale by antimycin A in the dark. The analysis also revealed a similar effect or artifact in uninhibited samples using the same sample illuminated with saturating continuous light, the standard procedure in photoacoustics (PA). A psaE(-) mutant showed more inhibition in the dark by DBMIB and with far-red Light by HQNO, but less inhibition in the far-red light by myxothiazol than the WT. Under normal growth conditions, maximum ES for the psaE(-) mutant (38 +/- 2%) was similar to that of the WT (42 +/- 2%). However, under mild heat stress, maximum ES for the psaE(-) mutant dropped to 26% while the WT maximum ES stayed unchanged at 41%, within batch-to-batch variation. These results would indicate that the PsaE protein is not essential for PS I cyclic electron flow under our experimental conditions but plays a stabilizing role in the PS I complex under a mild thermal stress.