PIN proteins perform a rate-limiting function in cellular auxin efflux

被引：729

作者：

Petrásek, J

Mravec, J

Bouchard, R

Blakeslee, JJ

Abas, M

Seifertová, D

Wisniewska, J

Tadele, Z

Kubes, M

Covanová, M

Dhonukshe, P

Skupa, P

Benková, E

Perry, L

Krecek, P

Lee, OR

Fink, GR

Geisler, M

Murphy, AS

Luschnig, C

Zazímalová, E

Friml, J

机构：

[1] Acad Sci Czech Republ, Inst Expt Bot, CR-16502 Prague 6, Czech Republic

[2] Charles Univ, Fac Sci, Dept Plant Physiol, CR-12844 Prague 2, Czech Republic

[3] Univ Tubingen, Ctr Plant Mol Biol ZMBP, D-72076 Tubingen, Germany

[4] Univ Zurich, Inst Plant Biol, Zurich Basel Plant Sci Ctr, CH-8007 Zurich, Switzerland

[5] Purdue Univ, Dept Hort, W Lafayette, IN 47907 USA

[6] Agr Univ Vienna, Univ Nat Resources & Appl Life Sci, Inst Appl Genet & Cell Biol, A-1190 Vienna, Austria

[7] Inst Gen & Mol Biol, Dept Biotechnol, PL-87100 Torun, Poland

[8] Univ Bern, Inst Plant Sci, CH-3013 Bern, Switzerland

[9] Whitehead Inst Biomed Res, Cambridge, MA 02142 USA

[10] Masaryk Univ, Dept Funct Genom & Proteom, Lab Mol Plant Physiol, Brno 62500, Czech Republic

来源：

SCIENCE | 2006年 / 312卷 / 5775期

关键词：

D O I：

10.1126/science.1123542

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed ( PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport - related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.

引用

页码：914 / 918

页数：5