Rapid detection of Klebsiella pneumoniae from blood culture bottles by real-time PCR

被引：40

作者：

Kurupati, P

Chow, C

Kumarasinghe, G

Poh, CL

机构：

[1] Natl Univ Singapore, Fac Med, Dept Microbiol, Singapore 117597, Singapore

[2] Natl Univ Singapore Hosp, Dept Lab Med, Singapore 119074, Singapore

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 03期

关键词：

D O I：

10.1128/JCM.42.3.1337-1340.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A LightCycler real-time PCR hybridization probe-based assay which detects a partial Klebsiella pneumoniae 16S rRNA gene was developed for the rapid identification of K. pneumoniae directly from growth-positive blood culture bottles (BACTEC 9240 system) within 2 h. No cross-reactivity was observed with 65 negative-control blood cultures that grew bacteria other than K. pneumoniae and 48 negative blood cultures from double-blind experiments, thus demonstrating 100% specificity when compared to results of conventional biochemical characterization. The assay also showed 100% sensitivity, as it correctly identified all 142 positive-control blood cultures and 4 from double-blind trials.

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页码：1337 / 1340

页数：4

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