Anchoring of bovine Chromosomes 4, 6, 7, 10, and 14 linkage group telomeric ends via FISH analysis of lambda clones

被引:7
作者
Smith, TPL [1 ]
LopezCorrales, N [1 ]
Grosz, MD [1 ]
Beattie, CW [1 ]
Kappes, SM [1 ]
机构
[1] ARS,USDA,FT KEOGH LIVESTOCK & RANGE RES LAB,MILES CITY,MT 59301
关键词
D O I
10.1007/s003359900434
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report the placement of 34 new microsatellite (ms) markers, isolated from a lambda phage genomic clone library, on the bovine genetic map by linkage to published markers. Five of these markers lie at or near the ends of linkage groups and are used to establish chromosomal coverage and orientation. Fluorescence in situ hybridization (FISH) analysis demonstrates that the linkage groups on the U.S. Meat Animal Research Center (MARC) map extend to the telomeric region of Chromosomes (Chrs) 7 and 10. Linkage groups on Chrs 4, 6, and 14 appear to be less inclusive.
引用
收藏
页码:333 / 336
页数:4
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