Analysis of the polymer-antibody-antigen interaction in a capacitive immunosensor by FTIR difference spectroscopy

被引：20

作者：

Sibai, A ^{[1
]}

Elamri, K ^{[1
]}

Barbier, D ^{[1
]}

JaffrezicRenault, N ^{[1
]}

Souteyrand, E ^{[1
]}

机构：

[1] ECOLE CENT LYON,LAB PHISICOCHIM INTERFACES,F-69131 ECULLY,FRANCE

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1996年 / 31卷 / 1-2期

关键词：

polymer-antibody-antigen interaction; immunosensor; FTIR difference spectroscopy;

D O I：

10.1016/0925-4005(96)80028-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The building of successive layers in a capacitive immunosensor has been monitored by FTIR and electrical measurements simultaneously. The detailed analysis of the infrared spectra showed: (1) the interpenetration of the polymer membrane and the biospecies; (2) a change in the conformation of the polymer leading to interaction with the antibodies then antigens, and charge exchange, which explains the unexpected variations in the capacitive signal and (3) the electrical and optical measurements are correlated.

引用

页码：125 / 130

页数：6

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