Single-Molecule Tracking in Living Cells Using Single Quantum Dot Applications

被引：32

作者：

Baba, Koichi ^{[1
]}

Nishida, Kohji ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Med, Dept Ophthalmol, Suita, Osaka 5650871, Japan

来源：

THERANOSTICS | 2012年 / 2卷 / 07期

基金：

日本学术振兴会;

关键词：

single molecule tracking; quantum dot; membrane dynamics; neuroscience; nuclear pore complex; in vivo real-time tracking; FLUORESCENCE INTERMITTENCY; PARTICLE TRACKING; AQUEOUS-PHASE; SEMICONDUCTOR NANOCRYSTALS; INTRACELLULAR DELIVERY; SURFACE TRAFFICKING; GLYCINE RECEPTORS; BLINKING; NANOPARTICLES; DIFFUSION;

D O I：

10.7150/thno.3890

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Revealing the behavior of single molecules in living cells is very useful for understanding cellular events. Quantum dot probes are particularly promising tools for revealing how biological events occur at the single molecule level both in vitro and in vivo. In this review, we will introduce how single quantum dot applications are used for single molecule tracking. We will discuss how single quantum dot tracking has been used in several examples of complex biological processes, including membrane dynamics, neuronal function, selective transport mechanisms of the nuclear pore complex, and in vivo real-time observation. We also briefly discuss the prospects for single molecule tracking using advanced probes.

引用

页码：655 / 667

页数：13

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