Assessment of Rab11-FIP2 interacting proteins in vitro

被引：6

作者：

Ducharme, NA ^{[1
]}

Jin, M ^{[1
]}

Lapierre, LA ^{[1
]}

Goldenring, JR ^{[1
]}

机构：

[1] Vanderbilt Univ, Sch Med, Dept Cell & Dev Biol, Nashville, TN 37212 USA

来源：

GTPASES REGULATING MEMBRANE TARGETING AND FUSION | 2005年 / 403卷

关键词：

D O I：

10.1016/S0076-6879(05)03061-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Members of the Rab family of small GTPases are involved in multiple trafficking events in both endocytotic and biosynthetic pathways. To understand more fully the regulation of these events, a concerted effort is underway to ascertain the binding partners and regulators of Rabs. Here, we describe methods to assess binding of Rab11a with Rab11-FIP2 and other Rab11-FIPs utilizing a modified far-Western approach. We then broaden this application to assess binding of Rab11-FIP2 with myosin Vb and homodimerization of Rab11-FIP2.

引用

页码：706 / 715

页数：10

共 22 条

[1] Apical recycling systems regulate directional budding of respiratory syncytial virus from polarized epithelial cells [J].

Brock, SC ;

Goldenring, JR ;

Crowe, JE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (25) :15143-15148

[2] Association of Rab25 and Rab11a with the apical recycling system of polarized Madin-Darby canine kidney cells [J].

Casanova, JE ;

Wang, XY ;

Kumar, R ;

Bhartur, SG ;

Navarre, J ;

Woodrum, JE ;

Altschuler, Y ;

Ray, GS ;

Goldenring, JR .

MOLECULAR BIOLOGY OF THE CELL, 1999, 10 (01) :47-61

[3] Rab11-FIP2, an adaptor protein connecting cellular components involved in internalization and recycling of epidermal growth factor receptors [J].

Cullis, DN ;

Philip, B ;

Baleja, JD ;

Feig, LA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (51) :49158-49166

[4] Rab11-family interacting protein 2 and myosin Vb are required for CXCR2 recycling and receptor-mediated chemotaxis [J].

Fan, GH ;

Lapierre, LA ;

Goldenring, JR ;

Sai, JQ ;

Richmond, A .

MOLECULAR BIOLOGY OF THE CELL, 2004, 15 (05) :2456-2469

[5] Differential regulation of CXCR2 trafficking by Rab GTPases [J].

Fan, GH ;

Lapierre, LA ;

Goldenring, JR ;

Richmond, A .

BLOOD, 2003, 101 (06) :2115-2124

[6] Rab11 is associated with transferrin-containing recycling compartments in K562 cells [J].

Green, EG ;

Ramm, E ;

Riley, NM ;

Spiro, DJ ;

Goldenring, JR ;

WesslingResnick, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 239 (02) :612-616

[7] Identification and characterization of a family of Rab11-interacting proteins [J].

Hales, CM ;

Griner, R ;

Hobdy-Henderson, KC ;

Dorn, MC ;

Hardy, D ;

Kumar, R ;

Navarre, J ;

Chan, EKL ;

Lapierre, LA ;

Goldenring, JR .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (42) :39067-39075

[8] Rab11 family interacting protein 2 associates with myosin Vb and regulates plasma membrane recycling [J].

Hales, CM ;

Vaerman, JP ;

Goldenring, JR .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (52) :50415-50421

[9] Rab11 is associated with GLUT4-containing vesicles and redistributes in response to insulin [J].

Kessler, A ;

Tomas, E ;

Immler, D ;

Meyer, HE ;

Zorzano, A ;

Eckel, J .

DIABETOLOGIA, 2000, 43 (12) :1518-1527

[10] Myosin Vb is associated with plasma membrane recycling systems [J].

Lapierre, LA ;

Kumar, R ;

Hales, CM ;

Navarre, J ;

Bhartur, SG ;

Burnette, JO ;

Provance, DW ;

Mercer, JA ;

Bähler, M ;

Goldenring, JR .

MOLECULAR BIOLOGY OF THE CELL, 2001, 12 (06) :1843-1857

← 1 2 3 →