Non-symmetrical cytosine methylation in tobacco pollen DNA

被引：36

作者：

Oakeley, EJ ^{[1
]}

Jost, JP ^{[1
]}

机构：

[1] FRIEDRICH MIESCHER INST,CH-4002 BASEL,SWITZERLAND

来源：

PLANT MOLECULAR BIOLOGY | 1996年 / 31卷 / 04期

关键词：

DNA methylation; auxin-binding protein; genomic sequencing; Nicotiana tabacum;

D O I：

10.1007/BF00019481

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have detected sequence-specific non-symmetrical cytosine methylation within a 140 bp region of the promoter for the tobacco auxin-binding protein gene T85 in pollen DNA. Direct sequencing of the population of bisulphite reaction products showed that, in this region, 10 out of a possible 49 cytosine residues were methylated at a high frequency in pollen whereas the corresponding region from somatic cells (leaf DNA) did not show a detectable level of methylation. The context of these sites was 1 x m(5)CpTpC, 1 x m(5)CpGpT, 1 x m(5)CpCpT, 2 x m(5)CpTpT, 2 x m(5)CpGpG, and 3 x m(5)CpApT of which only m(5)CpGpG and m(5)CpGpT fitted the consensus sequence for symmetrical methylation in plants.

引用

页码：927 / 930

页数：4

共 11 条

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Doyle J. J., 1990, Focus, V12, P13, DOI DOI 10.2307/2419362

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