Antioxidant and immunostimulating activities in vitro of sulfated polysaccharides isolated from Gracilaria rubra

被引：138

作者：

Di, Tong ^{[1
]}

Chen, Guijie ^{[1
]}

Sun, Yi ^{[1
]}

Ou, Shiyi ^{[2
]}

Zeng, Xiaoxiong ^{[1
]}

Ye, Hong ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China

[2] Jinan Univ, Dept Food Sci & Engn, Guangzhou 510632, Guangdong, Peoples R China

来源：

JOURNAL OF FUNCTIONAL FOODS | 2017年 / 28卷

基金：

中国国家自然科学基金;

关键词：

Gracilaria rubra; Polysaccharide; Characterization; Antioxidant activity; Immunostimulating activity; STRUCTURAL-CHARACTERIZATION; IMMUNOMODULATORY ACTIVITY; CHEMICAL-CHARACTERIZATION; EXTRACTION OPTIMIZATION; BIOLOGICAL-ACTIVITIES; ANTITUMOR-ACTIVITY; BROWN SEAWEED; MARINE-ALGAE; RED ALGAE; LEAVES;

D O I：

10.1016/j.jff.2016.11.005

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

In this work, crude polysaccharides from Gracilaria rubra (GRPS) and its three purified fractions, GRPS-1-1, GRPS-2-1 and GRPS-3-2, were obtained through hot water extraction and chromatographic purification with DEAE-52 cellulose column and Sephadex G-50 column. The three purified fractions, being homogeneous heteropolysaccharides with average molecular weights of 1310, 691, 923 kD and sulfuric radical contents of 5.96, 8.46 and 12.03%, respectively, were all composed of galactose and fucose. GRPS-2-1 and GRPS-3-2 had little toxicity to L-02 cells. Crude GRPS and its purified fractions exhibited strong scavenging activities against ABTS and superoxide radicals and lipid peroxidation inhibition. At a cellular level, GRPS-3-2 showed the strongest protective effect on H2O2-induced oxidative injury in PC12 cells. Moreover, GRPS-3-2 exhibited a much stronger immunostimulating activity on RAW264.7 cells than GRPS-1-1 and GRPS-2-1. The results suggested that GRPS, especially GRPS-3-2, could be developed as a promising functional food supplement. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：64 / 75

页数：12

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