Ultrastructural localisation of intramuscular expression of BDNF mRNA by silver-gold intensified non-radioactive in situ hybridisation

被引：34

作者：

Liem, RSB

Brouwer, N

Copray, JCVM

机构：

[1] Univ Groningen, Grad Sch Behav & Cognit Neurosci, Dept Cell Biol, Sect Electron Microscopy, NL-9713 EZ Groningen, Netherlands

[2] Univ Groningen, Grad Sch Behav & Cognit Neurosci, Dept Med Physiol, NL-9713 EZ Groningen, Netherlands

来源：

HISTOCHEMISTRY AND CELL BIOLOGY | 2001年 / 116卷 / 06期

关键词：

BDNF mRNA; in situ hybridisation; non-radioactive; silver-gold intensification; electron microscopy; soleus muscle; rat;

D O I：

10.1007/s00418-001-0349-z

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A non-radioactive in situ hybridisation method is described for the detection of low intramuscular levels of brain-derived neurotrophic factor (BDNF) mRNA at the electron microscope level. Application of high-grade silver-gold intensification of the diaminobenzidine end product of in situ hybridisation revealed that in adult rat muscle the constitutive expression of muscular BDNF is confined to the myofibres; satellite cells, Schwann cells, endothelial cells, fibroblasts or axons do not appear to contribute to BDNF production in normal muscle. Although muscular BDNF is a neurotrophic factor for innervating motoneurons and supposedly released only at the motor endplates, the production of BDNF mRNA appears to occur along the entire length of the myofibres and is not confined to nuclei in the postsynaptic regions.

引用

页码：545 / 551

页数：7

共 33 条

[1] Contraction-induced muscle fiber damage is increased in soleus muscle of streptozotocin-diabetic rats and is associated with elevated expression of brain-derived neurotrophic factor mRNA in muscle fibers and activated satellite cells [J].

Copray, S ;

Liem, R ;

Brouwer, N ;

Greenhaff, P ;

Habens, F ;

Fernyhough, P .

EXPERIMENTAL NEUROLOGY, 2000, 161 (02) :597-608

[2] Brain-derived neurotrophic factor mRNA levels are up-regulated in hindlimb skeletal muscle of diabetic rats: Effect of denervation [J].

Fernyhough, P ;

Maeda, K ;

Tomlinson, DR .

EXPERIMENTAL NEUROLOGY, 1996, 141 (02) :297-303

[3] DETECTION OF THE NICOTINIC ACETYLCHOLINE-RECEPTOR ALPHA-SUBUNIT MESSENGER-RNA BY INSITU HYBRIDIZATION AT NEUROMUSCULAR-JUNCTIONS OF 15-DAY-OLD CHICK STRIATED MUSCLES [J].

FONTAINE, B ;

SASSOON, D ;

BUCKINGHAM, M ;

CHANGEUX, JP .

EMBO JOURNAL, 1988, 7 (03) :603-609

[4]

Frostick SP, 1998, MICROSURG, V18, P397, DOI 10.1002/(SICI)1098-2752(1998)18:7<397::AID-MICR2>3.0.CO

[5]

2-F

[6] DIFFERENTIAL EXPRESSION OF MESSENGER-RNAS FOR NEUROTROPHINS AND THEIR RECEPTORS AFTER AXOTOMY OF THE SCIATIC-NERVE [J].

FUNAKOSHI, H ;

FRISEN, J ;

BARBANY, G ;

TIMMUSK, T ;

ZACHRISSON, O ;

VERGE, VMK ;

PERSSON, H .

JOURNAL OF CELL BIOLOGY, 1993, 123 (02) :455-465

[7] HIGH-GRADE INTENSIFICATION OF THE END-PRODUCT OF THE DIAMINOBENZIDINE REACTION FOR PEROXIDASE HISTOCHEMISTRY [J].

GALLYAS, F ;

GORCS, T ;

MERCHENTHALER, I .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1982, 30 (02) :183-184

[8] SUPPRESSION OF THE ARGYROPHIL-III REACTION BY MERCAPTO COMPOUNDS - A PREREQUISITE FOR THE INTENSIFICATION OF CERTAIN HISTOCHEMICAL REACTIONS BY PHASICAL DEVELOPERS [J].

GALLYAS, F .

ACTA HISTOCHEMICA, 1982, 70 (01) :99-105

[9] SPATIAL AND TEMPORAL EXPRESSION OF ACETYLCHOLINE-RECEPTOR RNAS IN INNERVATED AND DENERVATED RAT SOLEUS MUSCLE [J].

GOLDMAN, D ;

STAPLE, J .

NEURON, 1989, 3 (02) :219-228

[10] COOPERATION BETWEEN THE PRODUCTS OF DIFFERENT NUCLEI IN HYBRID MYOTUBES PRODUCES LOCALIZED ACETYLCHOLINE-RECEPTOR CLUSTERS [J].

GORDON, H ;

RALSTON, E ;

HALL, ZW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (14) :6595-6598

← 1 2 3 4 →