Diversity of hypoviruses and other double-stranded RNAs in Cryphonectria parasitica in North America

被引：71

作者：

Peever, TL ^{[1
]}

Liu, YC ^{[1
]}

Milgroom, MG ^{[1
]}

机构：

[1] CORNELL UNIV,DEPT PLANT PATHOL,ITHACA,NY 14853

来源：

PHYTOPATHOLOGY | 1997年 / 87卷 / 10期

关键词：

biocontrol; Castanea dentata; Endothia parasitica; fungal virus; hypovirulence;

D O I：

10.1094/PHYTO.1997.87.10.1026

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Double-stranded (ds) RNAs in Cryphonectria parasitica were randomly sampled from nine subpopulations in North America using an antibody-based detection system for dsRNA. dsRNA was detected in 166 (28%) of a total of 595 C. parasitica isolates sampled by immunoblotting. Incidence of dsRNA infection within subpopulations ranged from 0% in samples from New Hampshire and Ontario to 100% in County Line, A II. Most of the dsRNAs sampled were approximately 9 to 13 kb in size. dsRNAs from 72 isolates analyzed by probing Northern blots with P-32-labeled dsRNAs were in one of three hybridization groups. One hybridization group was widespread throughout eastern North America, being found in New York, New Jersey, Maryland, West Virginia, Kentucky, and Michigan. These dsRNAs hybridized to dsRNA from the previously described C. parasitica isolate SR2 from Maryland and are referred to as SR2-type dsRNAs. The second hybridization group was found almost exclusively in Michigan. The Michigan dsRNAs cross-hybridized to Cryphonectria hypovirus 3-GH2 (CHV3-GH2) and are referred to as CHV3-type dsRNAs.

引用

页码：1026 / 1033

页数：8

共 43 条

[31] Intercontinental population structure of the chestnut blight fungus, Cryphonectria parasitica [J].