Functional cloning as a means to-identify Leishmania genes involved in drug resistance

被引：13

作者：

Clos, J ^{[1
]}

Choudhury, K ^{[1
]}

机构：

[1] Bernhard Nocht Inst Trop Med, D-20359 Hamburg, Germany

来源：

MINI-REVIEWS IN MEDICINAL CHEMISTRY | 2006年 / 6卷 / 02期

关键词：

Leishmania; drug resistance; functional cloning; complementation; cosmid;

D O I：

10.2174/138955706775476028

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Resistance to anti-leishmanial drugs is a mounting problem in high-endemicity regions of South Asia and, potentially, in the context of HIV-Leishmania coinfections in Southern Europe. The molecular basis for clinical drug resistance is still largely unknown. It is important, however, to identify all relevant drug resistance markers for further drug development and Cor epidemiological surveys. An elegant and powerful method to identify such drug resistance markers without bias is functional cloning, using cosmid-based genomic DNA libraries. This review discusses the merits and caveats of this approach.

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收藏

页码：123 / 129

页数：7

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