Electrospinning versus knitting: two scaffolds for tissue engineering of the aortic valve

被引:73
作者
Van Lieshout, MI [1 ]
Vaz, CM [1 ]
Rutten, MCM [1 ]
Peters, GWM [1 ]
Baaijens, FPT [1 ]
机构
[1] Eindhoven Univ Technol, Dept Biomed Engn, NL-5600 MB Eindhoven, Netherlands
关键词
polycaprolactone; knitting; electrospinning; aortic valve; tissue engineering; scaffold;
D O I
10.1163/156856206774879153
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Two types of scaffolds were developed for tissue engineering of the aortic valve; an electrospun valvular scaffold and a knitted valvular scaffold. These scaffolds were compared in a physiologic flow system and in a tissue-engineering process. In fibrin gel enclosed human myofibroblasts were seeded onto both types of scaffolds and cultured for 23 days under continuous medium perfusion. Tissue formation was evaluated by confocal laser scanning microscopy, histology and DNA quantification. Collagen formation was quantified by a hydroxyproline assay. When subjected to physiologic flow, the spun scaffold tore within 6 h, whereas the knitted scaffold remained intact. Cells proliferated well on both types of scaffolds, although the cellular penetration into the spun scaffold was poor. Collagen production, normalized to DNA content, was not significantly different for the two types of scaffolds, but seeding efficiency was higher for the spun scaffold, because it acted as a cell impermeable filter. The knitted tissue constructs showed complete cellular in-growth into the pores. An optimal scaffold seems to be a combination of the strength of the knitted structure and the cell-filtering ability of the spun structure.
引用
收藏
页码:77 / 89
页数:13
相关论文
共 40 条
[1]  
BRODMAN RF, 1990, J CARDIOVASC SURG, V31, P142
[2]   INVITRO FIBROBLAST SEEDING OF PROSTHETIC ANTERIOR CRUCIATE LIGAMENTS - A PRELIMINARY-STUDY [J].
BRODY, GA ;
EISINGER, M ;
ARNOCZKY, SP ;
WARREN, RF .
AMERICAN JOURNAL OF SPORTS MEDICINE, 1988, 16 (03) :203-208
[3]   A synthetic fiber-reinforced stentless heart valve [J].
Cacciola, G ;
Peters, GWM ;
Baaijens, FPT .
JOURNAL OF BIOMECHANICS, 2000, 33 (06) :653-658
[4]   A three-dimensional mechanical analysis of a stentless fibre-reinforced aortic valve prosthesis [J].
Cacciola, G ;
Peters, GWM ;
Schreurs, PJG .
JOURNAL OF BIOMECHANICS, 2000, 33 (05) :521-530
[5]   Polycaprolactone microparticles and their biodegradation [J].
Chen, DR ;
Bei, JZ ;
Wang, SG .
POLYMER DEGRADATION AND STABILITY, 2000, 67 (03) :455-459
[6]  
DE BAKEY M E, 1958, AMA Arch Surg, V77, P713
[7]   PHYSICOMECHANICAL PROPERTIES OF DEGRADABLE POLYMERS USED IN MEDICAL APPLICATIONS - A COMPARATIVE-STUDY [J].
ENGELBERG, I ;
KOHN, J .
BIOMATERIALS, 1991, 12 (03) :292-304
[8]  
Gao JM, 1998, J BIOMED MATER RES, V42, P417, DOI 10.1002/(SICI)1097-4636(19981205)42:3<417::AID-JBM11>3.3.CO
[9]  
2-N
[10]  
Hoerstrup SP, 2000, CIRCULATION, V102, P44