Lipid-Gold-Nanoparticle Hybrid-Based Gene Delivery

被引：55

作者：

Rhim, Won-Kyu ^{[1
]}

Kim, Jin-Soo ^{[1
]}

Nam, Jwa-Min ^{[1
]}

机构：

[1] Seoul Natl Univ, Dept Chem, Seoul 151747, South Korea

来源：

SMALL | 2008年 / 4卷 / 10期

关键词：

gene delivery; gold; lipids; nanoparticles; non-viral vectors;

D O I：

10.1002/smll.200800628

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Lipid-DNA-AuNP (L-DNA-AuNP) hybrid-based gene delivery system was devised for better transfection efficiency and reduced cytotoxicity by taking advantages of AuNPs and liposomes as gene-delivery vehicles. pDNA-AuNPs were modified with cationic liposomes to form L-pDNA-AuNP hybrids and its structures were characterized using a cryo-transmission electron microscope (cryo-TEM). The transfection results show that the lipid-AuNP hybrid system has much better transfection efficiency than the liposome-based system. Dehydrogenases in viable cells induce a change in color, while liposome-based transfection system shows the highest cytotoxicity. It is also seen that only the L-pDNA-AuNP hybrid-based gene delivery system has both high delivery efficiency and low cytotoxicity. Less lipid is needed in the L-pDNA-AuNP system for efficient pDNA deliver than the lipid-pDNA system.

引用

页码：1651 / 1655

页数：5

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