Phosphorylation of Nlp by Plk1 negatively regulates its dynein-dynactin-dependent targeting to the centrosome

被引:77
作者
Casenghi, M [1 ]
Barr, FA [1 ]
Nigg, EA [1 ]
机构
[1] Max Planck Inst Biochem, Dept Cell Biol, D-82152 Martinsried, Germany
关键词
dynein; dynactin; Plk1; Nlp; centrosome;
D O I
10.1242/jcs.02622
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
When cells enter mitosis the microtubule (MT) network undergoes a profound rearrangement, in part due to alterations in the MT nucleating and anchoring properties of the centrosome. Ninein and the ninein-like protein (NIp) are centrosomal proteins involved in MT organisation in interphase cells. We show that the overexpression of these two proteins induces the fragmentation of the Golgi, and causes lysosomes to disperse toward the cell periphery. The ability of NIp and ninein to perturb the cytoplasmic distribution of these organelles depends on their ability to interact with the dynein-dynactin motor complex. Our data also indicate that dynactin is required for the targeting of NIp and ninein to the centrosome. Furthermore, phosphorylation of NIp by the polo-like kinase 1 (PIkl) negatively regulates its association with dynactin. These findings uncover a mechanism through which PIkl helps to coordinate changes in MT organisation with cell cycle progression, by controlling the dynein-dynactin-dependent transport of centrosomal proteins.
引用
收藏
页码:5101 / 5108
页数:8
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