Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction

被引：9

作者：

Corman, V. M. ^{[1
]}

Eckerle, I. ^{[1
]}

Bleicker, T. ^{[1
]}

Zaki, A. ^{[2
]}

Landt, O. ^{[3
]}

Eschbach-Bludau, M. ^{[1
]}

van Boheemen, S. ^{[4
]}

Gopal, R. ^{[5
]}

Ballhause, M. ^{[3
]}

Bestebroer, T. M. ^{[4
]}

Muth, D. ^{[1
]}

Mueller, M. A. ^{[1
]}

Drexler, J. F. ^{[1
]}

Zambon, M. ^{[5
]}

Osterhaus, A. D. ^{[4
]}

Fouchier, R. M. ^{[4
]}

Drosten, C. ^{[1
]}

机构：

[1] Univ Bonn, Med Ctr, Inst Virol, Bonn, Germany

[2] Dr Soliman Fakeeh Hosp, Virol Lab, Jeddah, Saudi Arabia

[3] TibMolbiol, Berlin, Germany

[4] Erasmus MC, Dept Virol & Virosci, Rotterdam, Netherlands

[5] Hlth Protect Agcy, London, England

来源：

EUROSURVEILLANCE | 2012年 / 17卷 / 39期

关键词：

ACUTE RESPIRATORY SYNDROME; SARS; ASSAY;

D O I：

暂无

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF) 1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5-6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.

引用

页码：3 / 8

页数：6