Acidic peptide hydrogel scaffolds enhance calcium phosphate mineral turnover into bone tissue

被引：51

作者：

Amosi, Nadav ^{[1
]}

Zarzhitsky, Shlomo ^{[1
,2
]}

Gilsohn, Eliezer ^{[1
]}

Salnikov, Olga ^{[3
,4
]}

Monsonego-Ornan, Efrat ^{[3
]}

Shahar, Ron ^{[4
]}

Rapaport, Hanna ^{[1
,2
]}

机构：

[1] Ben Gurion Univ Negev, Dept Biotechnol Engn, IL-81405 Beer Sheva, Israel

[2] Ben Gurion Univ Negev, Ilse Katz Ctr Meso & Nanoscale Sci & Technol, IL-81405 Beer Sheva, Israel

[3] Hebrew Univ Jerusalem, Sch Nutr, Robert H Smith Fac Agr Food & Environm, IL-76100 Rehovot, Israel

[4] Hebrew Univ Jerusalem, Koret Sch Vet Med, Robert H Smith Fac Agr Food & Environm, IL-76100 Rehovot, Israel

来源：

ACTA BIOMATERIALIA | 2012年 / 8卷 / 07期

关键词：

Amphiphilic peptides; Hydrogel scaffold; Biomineralization; Tricalcium phosphate; Bone healing; BETA-TRICALCIUM PHOSPHATE; IN-VIVO; SHEET PEPTIDES; REGENERATION; DEGRADATION; MATRICES; BIOMINERALIZATION; HYDROXYAPATITE; BIOMATERIALS; STIMULATION;

D O I：

10.1016/j.actbio.2012.04.003

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Designed peptides may generate molecular scaffolds in the form of hydrogels to support tissue regeneration. We studied the effect of hydrogels comprising beta-sheet-forming peptides rich in aspartic amino acids and of tricalcium phosphate (beta-TCP)-loaded hydrogels on calcium adsorption and cell culture in vitro, and on bone regeneration in vivo. The hydrogels were found to act as efficient depots for calcium ions, and to induce osteoblast differentiation in vitro. In vivo studies on bone defect healing in rat distal femurs analyzed by microcomputerized tomography showed that the peptide hydrogel itself induced better bone regeneration in comparison to non-treated defects. A stronger regeneration capacity was obtained in bone defects treated with beta-TCP-loaded hydrogels, indicating that the peptide hydrogels and the mineral act synergistically to enhance bone regeneration. In vivo regeneration was found to be better with hydrogels loaded with porous beta-TCP than with hydrogels loaded with non-porous mineral. It is concluded that biocompatible and biodegradable matrices, rich in anionic moieties that efficiently adsorb calcium ions while supporting cellular osteogenic activity, may efficiently promote beta-TCP turnover into bone mineral. (C) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：2466 / 2475

页数：10

共 40 条

[1]

INTERACTIONS BETWEEN ACIDIC PROTEINS AND CRYSTALS - STEREOCHEMICAL REQUIREMENTS IN BIOMINERALIZATION [J].