Photo-crosslinkable microcapsules formed by polyelectrolyte copolymer and modified collagen for rat hepatocyte encapsulation

被引：43

作者：

Quek, CH

Li, J

Sun, T

Ling, M

Chan, H

Mao, HQ

Gan, LM

Leong, KW

Yu, H

机构：

[1] Inst Mat Res & Engn, Singapore 117602, Singapore

[2] Johns Hopkins Univ, Dept Biomed Engn, Baltimore, MD 21205 USA

[3] Johns Hopkins Singapore, Singapore 117597, Singapore

[4] Natl Univ Singapore, Dept Physiol, Fac Med, Singapore 117597, Singapore

[5] Natl Univ Singapore, Fac Engn, Div Bioengn, Singapore 117576, Singapore

来源：

BIOMATERIALS | 2004年 / 25卷 / 17期

关键词：

photo-crosslinking; microcapsule; cell-encapsulation; polyelectrolyte; collagen; hepatocyte;

D O I：

10.1016/j.biomaterials.2003.09.112

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

New anionic polyelectrolyte tetra-copolymers with photo-crosslinkable 4-(4-methoxycinnamoyl)phenyl methacrylate monomer in addition to a HEMA-MMA-MAA ter-copolymer system were synthesized. The tetra-copolymers were used to form photo-crosslinkable microcapsules with modified collagen by complex coacervation for rat hepatocytes encapsulation. The hepatocytes were encapsulated within a two-layered membrane comprising of modified collagen as the inner core and an outer photo-crosslinkable copolymer shell. Upon photo-crosslinking of the microcapsules with UV-Vis light irradiation, the mechanical strength and chemical stability of the microcapsules, and the cellular functions of the encapsulated hepatocytes were enhanced. Particularly, the mechanical stability of the microcapsules was dramatically strengthened. The new photo-crosslinkable tetra-copolymer formulation described in this article has opened a way to the development of hepatocyte microencapsulation technology for bioartifical liver assist device. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：3531 / 3540

页数：10

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