A rapid selection/amplification procedure for high-level expression of recombinant protein in a metal-amplifiable mammalian expression system

被引：8

作者：

Bailey, CG ^{[1
]}

Baig, M ^{[1
]}

Gray, PP ^{[1
]}

Sunstrom, NA ^{[1
]}

机构：

[1] Univ New S Wales, Dept Biotechnol, CRC Biopharmaceut Res, Sydney, NSW 2052, Australia

来源：

BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 09期

关键词：

amplification; Chinese hamster ovary cells; metallothionein; recombinant protein expression; selection;

D O I：

10.1023/A:1008926708028

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a strategy for the selection and amplification of foreign gene expression in Chinese hamster ovary (CHO) cells employing a metallothionein gene-containing expression vector. This report describes an amplification procedure that results in an enrichment of clones exhibiting high levels of recombinant protein production and reduces the labour required for screening recombinant cell lines.

引用

页码：615 / 619

页数：5

共 10 条

[1]

ALT FW, 1978, J BIOL CHEM, V253, P1357

[2] AMPLIFICATION OF THE METALLOTHIONEIN-I GENE IN CADMIUM-RESISTANT MOUSE CELLS [J].

BEACH, LR ;

PALMITER, RD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (04) :2110-2114

[3] DNA-REPAIR IN AN ACTIVE GENE - REMOVAL OF PYRIMIDINE DIMERS FROM THE DHFR GENE OF CHO CELLS IS MUCH MORE EFFICIENT THAN IN THE GENOME OVERALL [J].

BOHR, VA ;

SMITH, CA ;

OKUMOTO, DS ;

HANAWALT, PC .

CELL, 1985, 40 (02) :359-369

[4] HIGH-LEVEL EXPRESSION OF TISSUE INHIBITOR OF METALLOPROTEINASES IN CHINESE HAMSTER OVARY CELLS USING GLUTAMINE-SYNTHETASE GENE AMPLIFICATION [J].

COCKETT, MI ;

BEBBINGTON, CR ;

YARRANTON, GT .

BIO-TECHNOLOGY, 1990, 8 (07) :662-667

[5] GENE REACTIVATION - A TOOL FOR THE ISOLATION OF MAMMALIAN DNA METHYLATION MUTANTS [J].

GOUNARI, F ;

BANKS, GR ;

KHAZAIE, K ;

JEGGO, PA ;

HOLLIDAY, R .

GENES & DEVELOPMENT, 1987, 1 (09) :899-912

[6] HIGH-FREQUENCY INDUCTION BY 5-AZACYTIDINE OF PROLINE INDEPENDENCE IN CHO-K1 CELLS [J].

HARRIS, M .

SOMATIC CELL AND MOLECULAR GENETICS, 1984, 10 (06) :615-624

[7] HUMAN METALLOTHIONEIN GENES - PRIMARY STRUCTURE OF THE METALLOTHIONEIN-II GENE AND A RELATED PROCESSED GENE [J].

KARIN, M ;

RICHARDS, RI .

NATURE, 1982, 299 (5886) :797-802

[8]

KAUFMAN RJ, 1990, METHOD ENZYMOL, V185, P537, DOI 10.1016/0076-6879(90)85044-O

[9] HYPERINDUCIBLE GENE-EXPRESSION FROM A METALLOTHIONEIN PROMOTER CONTAINING ADDITIONAL METAL-RESPONSIVE ELEMENTS [J].

MCNEALL, J ;

SANCHEZ, A ;

GRAY, PP ;

CHESTERMAN, CN ;

SLEIGH, MJ .

GENE, 1989, 76 (01) :81-88

[10] 5-AZACYTIDINE-INDUCED CONVERSION TO CADMIUM RESISTANCE CORRELATES WITH EARLY S-PHASE REPLICATION OF INACTIVE METALLOTHIONEIN GENES IN SYNCHRONIZED CHO CELLS [J].

STALLINGS, RL ;

CRAWFORD, BD ;

TOBEY, RA ;

TESMER, J ;

HILDEBRAND, CE .

SOMATIC CELL AND MOLECULAR GENETICS, 1986, 12 (05) :423-432

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