Re-scan confocal microscopy: scanning twice for better resolution

被引：183

作者：

De Luca, Giulia M. R. ^{[1
]}

Breedijk, Ronald M. P. ^{[1
]}

Brandt, Rick A. J. ^{[1
]}

Zeelenberg, Christiaan H. C. ^{[1
]}

de Jong, Babette E. ^{[1
]}

Timmermans, Wendy ^{[4
]}

Azar, Leila Nahidi ^{[5
]}

Hoebe, Ron A. ^{[6
]}

Stallinga, Sjoerd ^{[2
]}

Manders, Erik M. M. ^{[1
,3
]}

机构：

[1] Univ Amsterdam, Van Leeuwenhoek Ctr Adv Microscopy, Swammerdam Inst Life Sci, Amsterdam, Netherlands

[2] Delft Univ Technol, Quantitat Imaging Grp, Dept Imaging Sci & Technol, Delft, Netherlands

[3] Univ Amsterdam, Nikon Ctr Excellence Super Resolut Microscopy Dev, Amsterdam, Netherlands

[4] Univ Amsterdam, Swammerdam Inst Life Sci, Ctr NeuroSci, Amsterdam, Netherlands

[5] Nederlands Canc Inst NKI AV, Dept Cell Biol, Amsterdam, Netherlands

[6] Univ Amsterdam, Acad Med Ctr, Dept Cell Biol & Histol, Van Leeuwenhoek Ctr Adv Microscopy, NL-1105 AZ Amsterdam, Netherlands

来源：

BIOMEDICAL OPTICS EXPRESS | 2013年 / 4卷 / 11期

关键词：

STRUCTURED ILLUMINATION MICROSCOPY; FLUORESCENCE MICROSCOPY; SUPERRESOLUTION; DETECTOR; CAMERA;

D O I：

10.1364/BOE.4.002644

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

We present a new super-resolution technique, Re-scan Confocal Microscopy (RCM), based on standard confocal microscopy extended with an optical (re-scanning) unit that projects the image directly on a CCD-camera. This new microscope has improved lateral resolution and strongly improved sensitivity while maintaining the sectioning capability of a standard confocal microscope. This simple technology is typically useful for biological applications where the combination high-resolution and high sensitivity is required. (C) 2013 Optical Society of America

引用

页码：2644 / 2656

页数：13

共 18 条

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