Quantitative analysis of short-chain acyl-coenzymeAs in plant tissues by LC-MS-MS electrospray ionization method

被引：32

作者：

Perera, M. Ann D. N. ^{[2
]}

Choi, Suh-Yeon ^{[3
,4
]}

Wurtele, Eve Syrkin ^{[3
,4
]}

Nikolau, Basil J. ^{[1
,2
,3
]}

机构：

[1] Iowa State Univ, Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USA

[2] Iowa State Univ, WM Keck Metabol Res Lab, Ames, IA 50011 USA

[3] Iowa State Univ, Interdepartmental Genet Program, Ames, IA 50011 USA

[4] Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA 50011 USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 5-6期

基金：

美国国家科学基金会;

关键词：

Coenzyme A; Acyl-CoA; LC-MS-MS; Plants; Metabolites; TANDEM MASS-SPECTROMETRY; MALONYL-COA; ACETYL-COA; A ESTERS; QUANTIFICATION; ARABIDOPSIS; CARBOXYLASE; THIOESTERS;

D O I：

10.1016/j.jchromb.2008.12.053

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Because acyl-CoAs play major roles in numerous anabolic and catabolic pathways, the quantitative determination of these metabolites in biological tissues is paramount to understanding the regulation of these metabolic processes. Here, we report a method for the analysis of a collection of short-chain acyl-CoAs (<6 carbon chain length) from plant extracts. Identification of each individual acyl-CoA was conducted by monitoring specific mass-fragmentation ions that are derived from common chemical moieties of all Coenzyme A (CoA) derivatives, namely the adenosine triphosphate nucleotid, pantothenate and acylated cysteamine. This method is robust and quick, enabling the quantitative analysis of up to 12 different acyl-CoAs in plant metabolite extracts with minimal post-extraction processing, using a 30 min chromatographic run-time. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：482 / 488

页数：7

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