Dissociation behavior of doubly-charged tryptic peptides: Correlation of gas-phase cleavage abundance with Ramachandran plots

被引：62

作者：

Huang, YY

Triscari, JM

Pasa-Tolic, L

Anderson, GA

Lipton, MS

Smith, RD

Wysocki, VH ^{[1
]}

机构：

[1] Univ Arizona, Dept Chem, Tucson, AZ 85721 USA

[2] Pacific NW Natl Lab, Richland, WA 99352 USA

[3] Sci Applicat Int Corp, Tucson, AZ 85711 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 10期

关键词：

D O I：

10.1021/ja038041t

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Analysis of fragmentation patterns from 5654 unique doubly charged tryptic peptides is obtained. Great variability of average relative abundance of bond cleavage is found between different amino acid combinations. There exist similarities as well as differences between b and y ions. Strong enhancement or suppression of cleavage gives insight into possible chemical interactions at reactive conformations formed by preferred phi?psi angles. Copyright © 2004 American Chemical Society.

引用

收藏

页码：3034 / 3035

页数：2

相关论文

共 28 条

[1] Proton affinities of methyl esters of N-acetylated amino acids [J].

Addario, V ;

Guo, YZ ;

Chu, IK ;

Ling, Y ;

Ruggerio, G ;

Rodriquez, CF ;

Hopkinson, AC ;

Siu, KWM .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2002, 219 (01) :101-114

[2]

BRECI L, UNPUB

[3] Cleavage N-terminal to proline: Analysis of a database of peptide tandem mass spectra [J].

Breci, LA ;

Tabb, DL ;

Yates, JR ;

Wysocki, VH .

ANALYTICAL CHEMISTRY, 2003, 75 (09) :1963-1971

[4] Influence of peptide composition, gas-phase basicity, and chemical modification on fragmentation efficiency: Evidence for the mobile proton model [J].

Dongre, AR ;

Jones, JL ;

Somogyi, A ;

Wysocki, VH .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (35) :8365-8374

[5] AN APPROACH TO CORRELATE TANDEM MASS-SPECTRAL DATA OF PEPTIDES WITH AMINO-ACID-SEQUENCES IN A PROTEIN DATABASE [J].

ENG, JK ;

MCCORMACK, AL ;

YATES, JR .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1994, 5 (11) :976-989

[6] Selective gas-phase cleavage at the peptide bond terminal to aspartic acid in fixed-charge derivatives of asp-containing peptides [J].

Gu, CG ;

Tsaprailis, G ;

Breci, L ;

Wysocki, VH .

ANALYTICAL CHEMISTRY, 2000, 72 (23) :5804-5813

[7] The influence of histidine on cleavage C-terminal to acidic residues in doubly protonated tryptic peptides [J].

Huang, YY ;

Wysocki, VH ;

Tabb, DL ;

Yates, JR .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2002, 219 (01) :233-244

[8] Mining a tandem mass spectrometry database to determine the trends and global factors influencing peptide fragmentation [J].

Kapp, EA ;

Schütz, F ;

Reid, GE ;

Eddes, JS ;

Moritz, RL ;

O'Hair, RAJ ;

Speed, TP ;

Simpson, RJ .

ANALYTICAL CHEMISTRY, 2003, 75 (22) :6251-6264

[9] Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search [J].

Keller, A ;

Nesvizhskii, AI ;

Kolker, E ;

Aebersold, R .

ANALYTICAL CHEMISTRY, 2002, 74 (20) :5383-5392

[10] Phi/psi-chology: Ramachandran revisited [J].

Kleywegt, GJ ;

Jones, TA .

STRUCTURE, 1996, 4 (12) :1395-1400