In vivo study on the effects of curcumin on the expression profiles of anti-tumour genes (VEGF, CyclinD1 and CDK4) in liver of rats injected with DEN

被引：35

作者：

Huang, Chu Zhu ^{[1
]}

Huang, Wei Zhe ^{[2
]}

Zhang, Ge ^{[3
,4
]}

Tang, Dan Ling ^{[1
]}

机构：

[1] Shantou Univ, Coll Med, Affiliated Hosp 1, Dept Pharm, Shantou, Peoples R China

[2] Shantou Univ, Coll Med, Affiliated Hosp 1, Dept Cardiac Pulm Surg, Shantou, Peoples R China

[3] Chinese Univ Hong Kong, Dept Orthopaed, Shatin, Hong Kong, Peoples R China

[4] Chinese Univ Hong Kong, Dept Traumatol, Shatin, Hong Kong, Peoples R China

来源：

MOLECULAR BIOLOGY REPORTS | 2013年 / 40卷 / 10期

关键词：

Curcumin; Liver cancer; Rat; RT-PCR; CyclinD1; mRNA; VEGF mRNA; LIPID-PEROXIDATION; OXIDATIVE STRESS; DAMAGE; INHIBITION; MECHANISMS; DISEASE; BINDING;

D O I：

10.1007/s11033-013-2688-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

In this study we investigated the effects of curcumin, derived from plant Curcuma longa, on oxidative toxicity, and the possible molecular mechanism of antitumour of curcumin in liver cancer rats. Results showed that blood levels of Gamma-glutamyltransferase, aspartate aminotransferase, alanine aminotransferase, glutathione S-transferase, and liver level of MD were significantly decreased after curcumin feeding. Levels of the liver malondialdehyde MDA, nitric oxide and antioxidant enzymes were significantly increased. Moreover, RT-PCR and Western blot analysis results showed that curcumin treatment significantly decreased liver vascular endothelial growth factor (VEGF), CyclinD1 and CDK4 mRNA expression levels and CyclinD1 and CDK4 proteins levels in liver cancer rats. These findings were confirmed by histopathology. It is concluded that curcumin can protect the liver from the damage caused by N-nitrosodiethylamine. Moreover, curcumin has the potential to be used in a therapy for liver cancer. The present data provide evidence to support the presence of free radicals and VEGF, CyclinD1 and CDK4 mRNA in rat tumour cells. Studies are in progress in order to further characterize the role of VEGF, CyclinD1 and CDK4 mRNA in liver cancer cells and in hepatic therapeutics.

引用

页码：5825 / 5831

页数：7

共 32 条

[1]

Aggarwal BB, 2003, ANTICANCER RES, V23, P363

[2]

BEUTLER E, 1963, J LAB CLIN MED, V61, P882

[3]

BJARNASON GA, 1994, CIRCADIAN CANC THERA, P241

[4]

CARLBERG I, 1975, J BIOL CHEM, V250, P5475

[5]

Cole GM, 2007, ADV EXP MED BIOL, V595, P197

[6]

Through metal binding, curcumin protects against lead- and cadmium-induced lipid peroxidation in rat brain homogenates and against lead-induced tissue damage in rat brain [J].

Daniel, S ;

Limson, JL ;

Dairam, A ;

Watkins, GM ;

Daya, S .

JOURNAL OF INORGANIC BIOCHEMISTRY, 2004, 98 (02) :266-275

[7]

DRAPER HH, 1990, METHOD ENZYMOL, V186, P421

[8]

Chemopreventive and therapeutic effects of curcumin [J].

Duvoix, A ;

Blasius, R ;

Delhalle, S ;

Schnekenburger, M ;

Morceau, F ;

Henry, E ;

Dicato, M ;

Diederich, M .

CANCER LETTERS, 2005, 223 (02) :181-190

[9]

Ameliorating effect of curcumin on sodium arsenite-induced oxidative damage and lipid peroxidation in different rat organs [J].

El-Demerdash, Fatma M. ;

Yousef, Mokhtar I. ;

Radwan, Fatma M. E. .

FOOD AND CHEMICAL TOXICOLOGY, 2009, 47 (01) :249-254

[10]

PITUITARY FOLLICULAR CELLS SECRETE A NOVEL HEPARIN-BINDING GROWTH-FACTOR SPECIFIC FOR VASCULAR ENDOTHELIAL-CELLS [J].

FERRARA, N ;

HENZEL, WJ .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 161 (02) :851-858

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