Derivation of human embryonic stem cells in defined conditions

被引：761

作者：

Ludwig, TE

Levenstein, ME

Jones, JM

Berggren, WT

Mitchen, ER

Frane, JL

Crandall, LJ

Daigh, CA

Conard, KR

Piekarczyk, MS

Llanas, RA

Thomson, JA

机构：

[1] WiCell Res Inst, Madison, WI 53707 USA

[2] Genome Ctr Wisconsin, Madison, WI 53706 USA

[3] Univ Wisconsin, Wisconsin Natl Primate Res Ctr, Madison, WI 53715 USA

[4] Univ Wisconsin, Dept Obstet & Gynecol, Sch Med, Madison, WI 53715 USA

[5] Univ Wisconsin, Dept Anat, Sch Med, Madison, WI 53715 USA

来源：

NATURE BIOTECHNOLOGY | 2006年 / 24卷 / 02期

关键词：

D O I：

10.1038/nbt1177

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.

引用

页码：185 / 187

页数：3

共 15 条

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