Application of carbon fibre microelectrode for studying the impact of pesticides on a frog's egg

被引:15
作者
Fung, YS [1 ]
Mak, JLL [1 ]
机构
[1] Univ Hong Kong, Dept Chem, Hong Kong, Peoples R China
关键词
differential pulse voltammetry; carbon fibre ultramicroelectrode; organophosphorus pesticides; in vivo electrochemistry;
D O I
10.1016/S0013-4686(99)00093-6
中图分类号
O646 [电化学、电解、磁化学];
学科分类号
081704 ;
摘要
With the use of Differential Pulse Voltammetry (DPV), the carbon fibre microelectrode (30 micron diameter) encapsulated in a glass capillary with a 0.5 mm length exposed to solution was investigated as a tool to probe the entrance of electrochemically active pesticides into the frog's fertilized egg so as to study the baseline contamination and to assess the impact of environmental pollutants in water under changing environmental conditions. Frog's egg is a suitable biological system as its development from a single cell occurs in the natural environment with direct exposure to pesticides discharged to water bodies by human activities. Common pesticides such as parathion methyl, parathion ethyl, azinphos methyl and coumaphos were investigated using a carbon fibre microelectrode with optimized DPV conditions as follows: scan rate, 40 m V/s; pulse width, 12.5 ms, pulse amplitude, 100 ms and scan increment, 4 mV. Satisfactory results for the analytical performance were obtained showing detection limits at ppb range, good repeatability (RSD < 7%) and working ranges covering concentrations over three orders of magnitude for the pesticides studied. Results on the effect of various environmental conditions such as temperature, pollutants concentration and exposure time showed biological variation in the response of the frog's egg to pesticide exposure and its suitability as an effective biological indicator to provide a continuous in situ assessment of the environmental impact of pesticides. (C) 1999 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:3855 / 3864
页数:10
相关论文
共 23 条
  • [1] METHYLENE-BLUE PERFLUOROSULFONATED IONOMER MODIFIED MICROCYLINDER CARBON-FIBER ELECTRODE AND ITS APPLICATION FOR THE DETERMINATION OF HEMOGLOBIN
    CHEN, HY
    JU, HX
    XUN, YG
    [J]. ANALYTICAL CHEMISTRY, 1994, 66 (24) : 4538 - 4542
  • [2] ELECTROCHEMICAL STUDY AND DETERMINATION OF TODRALAZINE BY ADSORPTIVE STRIPPING VOLTAMMETRY
    DEBALUGERA, ZG
    BARRIO, RJ
    GOICOLEA, A
    ARRANZ, JF
    [J]. ELECTROANALYSIS, 1991, 3 (4-5) : 423 - 427
  • [3] ELECTROANALYTICAL APPLICATIONS OF CARBON-FIBER ELECTRODES
    EDMONDS, TE
    [J]. ANALYTICA CHIMICA ACTA, 1985, 175 (SEP) : 1 - 22
  • [4] ELLEN R, 1993, ANAL CHIM ACTA, V278, P5
  • [5] SIMPLE AND RELIABLE FABRICATION OF CARBON-FIBER ULTRAMICROELECTRODES
    FERNANDEZ, AIS
    CALZON, JAG
    GARCIA, AC
    BLANCO, PT
    [J]. ELECTROANALYSIS, 1991, 3 (4-5) : 413 - 417
  • [6] DIFFERENTIAL-PULSE ADSORPTIVE STRIPPING VOLTAMMETRY OF FOOD AND COSMETIC SYNTHETIC COLORING MATTERS AND THEIR DETERMINATION AND PARTIAL IDENTIFICATION IN TABLE COATINGS AND COSMETICS
    FOGG, AG
    BARROS, AA
    CABRAL, JO
    [J]. ANALYST, 1986, 111 (07) : 831 - 835
  • [7] Application of carbon fibre ultramicroelectrode for studying the impact of environmental pollutants on frog's egg
    Fung, YS
    Wong, CCW
    Deng, JQ
    [J]. ELECTROCHIMICA ACTA, 1997, 42 (20-22) : 3257 - 3264
  • [8] AMPEROMETRIC SENSORS FOR PEROXIDE, CHOLINE, AND ACETYLCHOLINE BASED ON ELECTRON-TRANSFER BETWEEN HORSERADISH-PEROXIDASE AND A REDOX POLYMER
    GARGUILO, MG
    HUYNH, N
    PROCTOR, A
    MICHAEL, AC
    [J]. ANALYTICAL CHEMISTRY, 1993, 65 (05) : 523 - 528
  • [9] GONON F, 1978, CR ACAD SCI D NAT, V286, P1203
  • [10] Hartung HP, 1996, BAILLIERE CLIN NEUR, V5, P1