Effect of high temperature on protein expression in strawberry plants

被引：31

作者：

Ledesma, NA ^{[1
]}

Kawabata, S ^{[1
]}

Sugiyama, N ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan

来源：

BIOLOGIA PLANTARUM | 2004年 / 48卷 / 01期

关键词：

Fragaria x ananassa; heat shock protein; immunoblotting;

D O I：

10.1023/B:BIOP.0000024278.62419.ee

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Strawberry plants (Fragaria x ananassa Duch.) cvs. Nyoho and Toyonoka were exposed to temperatures of 20, 33, and 42degreesC for 4 h, and protein patterns in leaves and flowers was analyzed by 2-dimensional polyacrylamide gel electrophoresis and immunoblotting. In leaves and flowers of both cultivars, the content of most proteins decreased, but a few new proteins appeared in response to heat stress. These heat shock proteins ( Hsps) were detected in the range of 19-29 kDa in leaves, and 16-26 kDa in flowers. The intensity of a 43 kDa protein spot increased in response to heat stress in Nyoho flowers, but not in Toyonoka flowers. The peaHsp17.7 antibody recognized one band at approximately 26 kDa in leaves, and two bands at approximately 16 and 17 kDa in flowers of both cultivars. These results show that the effects of heat stress on Hsp synthesis in strawberry plants differ between plant organs and between cultivars.

引用

页码：73 / 79

页数：7

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