A high γ-aminobutyric acid-producing Lactobacillus brevis isolated from Chinese traditional paocai

被引:83
作者
Li, Haixing [1 ]
Gao, Dandan [1 ]
Cao, Yusheng [1 ]
Xu, Hengyi [1 ]
机构
[1] Nanchang Univ, Sinogerman Joint Res Inst, Nanchang 330047, Peoples R China
关键词
paocai; lactic acid bacteria; gamma-aminobutyric acid; screening and identification; Lactobacillus brevis;
D O I
10.1007/BF03175570
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
To find a high gamma-aminobutyric acid-producing lactic acid bacterium, more than 1000 strains of lactic acid bacteria isolated from paocai samples in various areas of China were screened by the ability in production of gamma-aminobutyric acid, analysed with paper chromatography, HPLC and HPLC-MS. Among them, one strain NCL912 exhibited high ability to convert sodium glutamate to gamma-aminobutyric acid. The strain accumulated 149.05 mM of gamma-aminobutyric acid in a modified MRS medium containing 3% sodium glutamate after 48 h of static cultivation at 30 degrees C. This strain was identified as Lactobacillus brevis according to its phenotypic and phylogenetic characteristics.
引用
收藏
页码:649 / 653
页数:5
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