Chemical characteristic of an anticoagulant-active sulfated polysaccharide from Enteromorpha clathrata

被引：179

作者：

Qi, Xiaohui ^{[1
]}

Mao, Wenjun ^{[1
]}

Gao, Yan ^{[2
]}

Chen, Yin ^{[1
]}

Chen, Yanli ^{[1
]}

Zhao, Chunqi ^{[1
]}

Li, Na ^{[1
]}

Wang, Chunyan ^{[1
]}

Yan, Mengxia ^{[1
]}

Lin, Cong ^{[1
]}

Shan, Jimiao ^{[1
]}

机构：

[1] Ocean Univ China, Inst Marine Drug & Food, Minist Educ, Key Lab Marine Drugs, Qingdao 266003, Peoples R China

[2] Natl Oceanog Ctr Qingdao, Qingdao 266071, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2012年 / 90卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Enteromorpha clathrata; Sulfated polysaccharide; Chemical characteristic; Anticoagulant activity; HEPARIN-COFACTOR-II; ANTIOXIDANT PROPERTIES; GREEN-ALGA; GALACTAN;

D O I：

10.1016/j.carbpol.2012.07.077

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

A sulfated polysaccharide FEP from marine green alga Enteromorpha clathrata was extracted with hot water and further purified by ion-exchange and size-exclusion chromatography. Results of chemical and spectroscopic analyses showed that FEP was a high arabinose-containing sulfated polysaccharide with sulfate ester of 31.0%, and its average molecular weight was about 511 kDa. The backbone of FEP was mainly composed of (1 -> 4)-linked beta-L-arabinopyranose residues with partially sulfate groups at the C-3 position. In vitro anticoagulant assay indicated that FEP effectively prolonged the activated partial thromboplastin time and thrombin time. The investigation demonstrated that FEP was a novel sulfated polysaccharide with different chemical characteristics from other sulfated polysaccharides from marine algae, and could be a potential source of anticoagulant. (C) 2012 Elsevier Ltd. All rights reserved,

引用

页码：1804 / 1810

页数：7

共 35 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2]

Chemical structure and antiviral activity of the sulfated heterorhamnan isolated from the green seaweed Gayralia oxysperma [J].