Stereological length estimation using spherical probes

被引:150
作者
Mouton, PR
Gokhale, AM
Ward, NL
West, MJ
机构
[1] NIA, Neurosci Lab, Gerontol Res Ctr, NIH, Baltimore, MD 21224 USA
[2] Georgia Inst Technol, Sch Mat Sci & Engn, Atlanta, GA 30332 USA
[3] Sunnybrook & Womens Mol & Cellular Hlth Sci Ctr, Div Coll Biol, Toronto, ON, Canada
[4] Univ Aarhus, Inst Anat, Aarhus, Denmark
关键词
dentate gyrus; hippocampus; isotropic; space balls; stereology; unbiased;
D O I
10.1046/j.1365-2818.2002.01006.x
中图分类号
TH742 [显微镜];
学科分类号
摘要
Lineal structures in biological tissue support a wide variety of physiological functions, including membrane stabilization, vascular perfusion. and cell-to-cell communication. In 1953. Smith and Guttman demonstrated a stereological method to estimate the total length density (L-v) of linear objects based on random intersections with a two-dimensional sampling probe. Several methods have been developed to ensure the required isotropy of object-probe intersections, including isotropic-uniform-random (IUR) sections, vertical-uniform-random (VUR) slices, and isotropic virtual planes. The disadvantages of these methods are the requirements for inconvenient section orientations (IUR, VUR) or complex counting rules at multiple focal planes (isotropic virtual planes). To overcome these limitations we report a convenient and straightforward approach to estimate L-v and total length, L, for linear objects on tissue sections cut at any arbitrary orientation. The approach presented here uses spherical probes that are inherently isotropic, combined with unbiased fractionator Sampling, to demonstrate total L estimation for thin nerve fibres in dorsal hippocampus of the mouse brain.
引用
收藏
页码:54 / 64
页数:11
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