Hippocampal neuron and synaptophysin-positive bouton number in aging C57BL/6 mice

被引:199
作者
Calhoun, ME
Kurth, D
Phinney, AL
Long, JM
Hengemihle, J
Mouton, PR
Ingram, DK
Jucker, M
机构
[1] Univ Basel, Inst Pathol, CH-4003 Basel, Switzerland
[2] NIA, Gerontol Res Ctr, NIH, Baltimore, MD 21224 USA
关键词
aging; CNS; brain; mouse; hippocampus; neurons; synapses; stereology; maze learning; memory; behavior; synaptophysin;
D O I
10.1016/S0197-4580(98)00098-0
中图分类号
R592 [老年病学]; C [社会科学总论];
学科分类号
03 ; 0303 ; 100203 ;
摘要
A loss of hippocampal neurons and synapses had been considered a hallmark of normal aging and, furthermore, to br: a substrate of age-related learning and memory deficits. Recent stereological studies in humans have shown that only a relatively minor neuron loss occurs with aging and that this loss is restricted to specific brain regions, including hippocampal subregions. Here, we investigate these age-related changes in C57BL/6J mice, one of thr must commonly used laboratory mouse strains. Twenty-five mice (groups at 2, 14, and 28-31 months of age) were assessed for Morris water-maze performance, and modern stereological techniques were used to estimate total neuron and synaptophysin-positive bouton number in hippocampal subregions at the light microscopic level. Results revealed that performance in the water maze was largely maintained with aging. No age-related decline was observed in number of dentate gyrus granule cells or CAI pyramidal cells. In addition, no age-related change in number of synaptophysin-positive boutons was observed in the molecular layer of the dentate gyrus or CAI region of hippocampus. We observed a significant correlation between dentate gyrus synaptophysin-positive bouton number and water-maze performance. These results demonstrate that C57BL/6J mice do not exhibit major age-related deficits in spatial learning or hippocampal structure, providing a baseline for further study of mouse brain aging. (C) 1999 Elsevier Science Inc.
引用
收藏
页码:599 / 606
页数:8
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