Directed differentiation and neurite extension of mouse embryonic stem cell on aligned poly(lactide) nanofibers functionalized with YIGSR peptide

被引：129

作者：

Callahan, Laura A. Smith ^{[1
]}

Xie, Sibai ^{[1
]}

Barker, Ian A. ^{[2
]}

Zheng, Jukuan ^{[1
]}

Reneker, Darrell H. ^{[1
]}

Dove, Andrew P. ^{[2
]}

Becker, Matthew L. ^{[1
,3
]}

机构：

[1] Univ Akron, Dept Polymer Sci, Akron, OH 44325 USA

[2] Univ Warwick, Dept Chem, Coventry CV4 7AL, W Midlands, England

[3] Austen Bioinnovat Inst Akron, Ctr Biomat Med, Akron, OH 44308 USA

来源：

BIOMATERIALS | 2013年 / 34卷 / 36期

基金：

英国工程与自然科学研究理事会;

关键词：

Nanofiber; Neural differentiation; YIGSR; Polymer; Electrospinning; ELECTROSPUN NANOFIBERS; ADHESION; SURFACE; FIBERS; OUTGROWTH; POLY(L-LACTIDE); BIOMATERIALS; CHEMISTRY; DIAMETER; LAMININ;

D O I：

10.1016/j.biomaterials.2013.08.028

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

End-functional PLLA nanofibers were fabricated into mats of random or aligned fibers and functionalized post-spinning using metal-free "click chemistry" with the peptide Tyr-Ile-Gly-Ser-Arg (YIGSR). Fibers that were both aligned and functionalized with YIGSR were found to significantly increase the fraction of mouse embryonic stem cells (mESC) expressing neuron-specific class III beta-tubulin (TUJ1), the level of neurite extension and gene expression for neural markers compared to mESC cultured on random fiber mats and unfunctionalized matrices. Precise functionalization of degradable polymers with bioactive peptides created translationally-relevant materials that capitalize on the advantages of both synthetic and natural systems, while mitigating the classic limitations of each. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：9089 / 9095

页数：7

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