Detection and localization of syntrophic propionate-oxidizing bacteria in granular sludge by in situ hybridization using 16S rRNA-based oligonucleotide probes

被引:125
作者
Harmsen, HJM
Kengen, HMP
Akkermans, ADL
Stams, AJM
deVos, WM
机构
[1] AGR UNIV WAGENINGEN,DEPT MICROBIOL,6703 CT WAGENINGEN,NETHERLANDS
[2] CATHOLIC UNIV NIJMEGEN,DEPT EXPTL BOT,RES GRP PLANT CELL BIOL,6525 ED NIJMEGEN,NETHERLANDS
关键词
D O I
10.1128/AEM.62.5.1656-1663.1996
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In situ hybridization with fluorescent oligonucleotides was used to detect and localize microorganisms in the grannies of two lab-scale upflow anaerobic sludge blanket reactors that had been fed for several months with either sucrose or a mixture of volatile fatty acids, Sections of the granules were hybridized with 16S rRNA-targeted oligonucleotide probes for Bacteria, Archaea, specific phylogenetic groups of methanogens, and two syntrophic propionate-oxidizing strains, MPOB and KOPROP1. Cells of the syntrophic strain KOPROP1 were not detected in either type of sludge granules. Hybridizations of the sucrose-fed granules showed an outer layer of mainly bacterial microcolonies with different morphologies, More inwards of these granules, a layer of different methanogenic microcolonies mixed with large colonies of the syntrophic strain MPOB could be detected. The MPOB colonies were interwined with hydrogen- or formate-consuming methanogens, indicating their syntrophic growth, The granules fed with volatile fatty acids showed an outer layer of mainly bacteria acid then a thick layer of Methanosaeta-like methanogens mixed with a few bacteria and a layer of methanogens mixed with syntrophic MPOB microcolonies. The centers of both sludge types consisted of large cavities and methanogenic microcolonies. These results indicate a juxtapositioning of syntrophic bacteria acid methanogens and provide additional evidence for a layered microbial architecture of anaerobic granular sludge.
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页码:1656 / 1663
页数:8
相关论文
共 33 条
[11]  
HARMSEN HJM, 1993, ARCH MICROBIOL, V160, P238
[12]  
HEPPNER B, 1992, APPL MICROBIOL BIOT, V36, P810, DOI 10.1007/BF00172200
[13]  
JETTEN MSM, 1992, FEMS MICROBIOL LETT, V88, P181, DOI 10.1111/j.1574-6968.1992.tb04987.x
[14]   HETEROGENEOUS DISTRIBUTION OF MICROBIAL ACTIVITY IN METHANOGENIC AGGREGATES - PH AND GLUCOSE MICROPROFILES [J].
LENS, PNL ;
DEBEER, D ;
CRONENBERG, CCH ;
HOUWEN, FP ;
OTTENGRAF, SPP ;
VERSTRAETE, WH .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (11) :3803-3815
[15]   ANAEROBIC-DIGESTION AND WASTE-WATER TREATMENT SYSTEMS [J].
LETTINGA, G .
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 1995, 67 (01) :3-28
[16]   TOPOGRAPHY OF METHANOGENIC SUBPOPULATIONS IN A MICROBIAL CONSORTIUM ADAPTING TO THERMOPHILIC CONDITIONS [J].
MACARIO, AJL ;
VISSER, FA ;
VANLIER, JB ;
DEMACARIO, EC .
JOURNAL OF GENERAL MICROBIOLOGY, 1991, 137 :2179-2189
[17]  
MACINERNEY MJ, 1991, PROKARYOTES, P2048
[18]   LAYERED STRUCTURE OF BACTERIAL AGGREGATES PRODUCED IN AN UPFLOW ANAEROBIC SLUDGE BED AND FILTER REACTOR [J].
MACLEOD, FA ;
GUIOT, SR ;
COSTERTON, JW .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (06) :1598-1607
[19]   THE RIBOSOMAL DATABASE PROJECT [J].
MAIDAK, BL ;
LARSEN, N ;
MCCAUGHEY, MJ ;
OVERBEEK, R ;
OLSEN, GJ ;
FOGEL, K ;
BLANDY, J ;
WOESE, CR .
NUCLEIC ACIDS RESEARCH, 1994, 22 (17) :3485-3487
[20]  
PRENSIER G, 1988, GRANULAR ANAEROBIC S, P55