Expression of the vault RNA protects cells from undergoing apoptosis

被引：68

作者：

Amort, Melanie ^{[1
]}

Nachbauer, Birgit ^{[1
,2
,3
]}

Tuzlak, Selma ^{[4
]}

Kieser, Arnd ^{[5
,6
]}

Schepers, Aloys ^{[5
,6
]}

Villunger, Andreas ^{[4
]}

Polacek, Norbert ^{[1
,2
]}

机构：

[1] Med Univ Innsbruck, Div Genom & RNom, A-6020 Innsbruck, Austria

[2] Univ Bern, Dept Chem & Biochem, CH-3012 Bern, Switzerland

[3] Univ Bern, Grad Sch Cellular & Biomed Sci, CH-3012 Bern, Switzerland

[4] Med Univ Innsbruck, Div Dev Immunol, A-6020 Innsbruck, Austria

[5] Helmholtz Zentrum Munchen, Res Unit Gene Vectors, D-81377 Munich, Germany

[6] German Ctr Infect Res DZIF, D-81377 Munich, Germany

来源：

NATURE COMMUNICATIONS | 2015年 / 6卷

基金：

奥地利科学基金会; 瑞士国家科学基金会;

关键词：

EPSTEIN-BARR-VIRUS; CASPASE RECRUITMENT DOMAIN; NONCODING RNA; MULTIDRUG-RESISTANCE; MALIGNANT-CELLS; CANCER-CELLS; REPRESSOR; DEATH; TRANSCRIPTION; ACTIVATION;

D O I：

10.1038/ncomms8030

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Non-protein-coding RNAs are a functionally versatile class of transcripts exerting their biological roles on the RNA level. Recently, we demonstrated that the vault complex-associated RNAs (vtRNAs) are significantly upregulated in Epstein-Barr virus (EBV)-infected human B cells. Very little is known about the function(s) of the vtRNAs or the vault complex. Here, we individually express latent EBV-encoded proteins in B cells and identify the latent membrane protein 1 (LMP1) as trigger for vtRNA upregulation. Ectopic expression of vtRNA1-1, but not of the other vtRNA paralogues, results in an improved viral establishment and reduced apoptosis, a function located in the central domain of vtRNA1-1. Knockdown of the major vault protein has no effect on these phenotypes revealing that vtRNA1-1 and not the vault complex contributes to general cell death resistance. This study describes a NF-kappa B-mediated role of the non-coding vtRNA1-1 in inhibiting both the extrinsic and intrinsic apoptotic pathways.

引用

页数：11

共 57 条

[1]

Interaction of vault particles with estrogen receptor in the MCF-7 breast cancer cell [J].