Rat cytomegalovirus open reading frame R44 is an early-late gene that encodes a nuclear protein

被引：6

作者：

Kaptein, SJF ^{[1
]}

Beuken, E ^{[1
]}

Grauls, GELM ^{[1
]}

Bruggeman, CA ^{[1
]}

Vink, C ^{[1
]}

机构：

[1] Univ Maastricht, Dept Med Microbiol, Cardiovasc Res Inst Maastricht, NL-6202 AZ Maastricht, Netherlands

来源：

ARCHIVES OF VIROLOGY | 2001年 / 146卷 / 11期

关键词：

D O I：

10.1007/s007050170031

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Rat cytomegalovirus (RCMV) open reading frame R44 is the homolog of human cytomegalovirus gene UL44, which encodes the DNA polymerase accessory protein. Here, we show that R44 is transcribed as a 3.6-kb mRNA within the early and late phases of infection in vitro. In order to find potential monoclonal antibodies (MoAbs) directed against the R44-encoded protein (pR44), a panel of anti-RCMV MoAbs was screened for binding to pR44 recombinant proteins. Thus, an anti-pR44 MoAb, termed RCMV8, was identified. By using this MoAb, pR44 could be detected as early as 8 h after RCMV infection in vitro. The pR44 protein was determined to have a molecular mass of approximately 55 kDa and was found to be localized to the nucleus of RCMV-infected cells.

引用

页码：2211 / 2218

页数：8

共 35 条

[11] PURIFICATION OF THE HERPES-SIMPLEX VIRUS TYPE-1 65-KILODALTON DNA-BINDING PROTEIN - PROPERTIES OF THE PROTEIN AND EVIDENCE OF ITS ASSOCIATION WITH THE VIRUS-ENCODED DNA-POLYMERASE [J].