Making hybrids of two-hybrid systems

被引：10

作者：

Dagher, MC ^{[1
]}

FilholCochet, O ^{[1
]}

机构：

[1] CEA,DEPT BIOL MOL & STRUCT,INSERM,U244,LAB BIOCHIM REGULAT CELLULAIRES ENDOCRINES,F-38054 GRENOBLE 9,FRANCE

来源：

BIOTECHNIQUES | 1997年 / 22卷 / 05期

关键词：

CASEIN KINASE-II; PROTEIN-PROTEIN INTERACTIONS; 2-HYBRID SYSTEM; CATALYTIC ACTIVITY; ALPHA-SUBUNIT; BETA-SUBUNIT; ASSAY; RAS;

D O I：

10.2144/97225st05

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two-hybrid systems are powerful tools to find new partners for a protein interest. However; exchange of material between two-hybrid users has been handicapped by the various versions of two-hybrid systems available mid by the widely accepted idea that they are not compatible. In the present paper we show that, contrary to the dogma, the most often used two-hybrid systems may be combined by either transformation or mating assays. The protocol to be followed in each case is provided. This will greatly work of interacting proteins, by reconciling the ''two-hybrid systems'' and the ''interaction trap''.

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收藏

页码：916 / &

页数：5

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