Deriving folds of macromolecular complexes through electron cryomicroscopy and bioinformatics approaches

被引：32

作者：

Chiu, W ^{[1
]}

Baker, ML

Jiang, W

Zhou, ZH

机构：

[1] Baylor Coll Med, Program Struct & Computat Biol & Mol Biophys, Houston, TX 77030 USA

[2] Baylor Coll Med, Verna & Marrs McLean Dept Biochem & Mol Biol, Houston, TX 77030 USA

[3] Univ Texas, Houston Med Sch, Dept Pathol & Lab Med, Houston, TX 77030 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2002年 / 12卷 / 02期

关键词：

D O I：

10.1016/S0959-440X(02)00319-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Intermediate-resolution (7-9 Angstrom) structures of large macromolecular complexes can be obtained by electron cryomicroscopy. This structural information, combined with bioinformatics data for the individual protein components or domains, can lead to a fold model for the entire complex. Such approaches have been demonstrated with the 6.8 Angstrom structure of the rice dwarf virus to derive models for the major capsid shell proteins.

引用

页码：263 / 269

页数：7

共 47 条

[41] Completeness in structural genomics [J].

Vitkup, D ;

Melamud, E ;

Moult, J ;

Sander, C .

NATURE STRUCTURAL BIOLOGY, 2001, 8 (06) :559-566

[42] Quantitative fitting of atomic models into observed densities derived by electron microscopy [J].

Volkmann, N ;

Hanein, D .

JOURNAL OF STRUCTURAL BIOLOGY, 1999, 125 (2-3) :176-184

[43] The three-dimensional structure of aquaporin-1 [J].

Walz, T ;

Hirai, T ;

Murata, K ;

Heymann, JB ;

Mitsuoka, K ;

Fujiyoshi, Y ;

Smith, BL ;

Agre, P ;

Engel, A .

NATURE, 1997, 387 (6633) :624-627

[44] Situs: A package for docking crystal structures into low-resolution maps from electron microscopy [J].

Wriggers, W ;

Milligan, RA ;

McCammon, JA .

JOURNAL OF STRUCTURAL BIOLOGY, 1999, 125 (2-3) :185-195

[45] Modeling tricks and fitting techniques for multiresolution structures [J].

Wriggers, W ;

Chacón, P .

STRUCTURE, 2001, 9 (09) :779-788

[46] Seeing the herpesvirus capsid at 8.5 Å [J].

Zhou, ZH ;

Dougherty, M ;

Jakana, J ;

He, J ;

Rixon, FJ ;

Chiu, W .

SCIENCE, 2000, 288 (5467) :877-880

[47] Electron cryomicroscopy and bioinformatics suggest protein fold models for rice dwarf virus [J].

Zhou, ZH ;

Baker, ML ;

Jiang, W ;

Dougherty, M ;

Jakana, J ;

Dong, G ;

Lu, GY ;

Chiu, W .

NATURE STRUCTURAL BIOLOGY, 2001, 8 (10) :868-873

← 1 2 3 4 5 →