MicroRNA-25 functions as a potential tumor suppressor in colon cancer by targeting Smad7

被引：160

作者：

Li, Qiang ^{[2
]}

Zou, Chaoxia ^{[1
]}

Zou, Chendan ^{[1
]}

Han, Zhongjing ^{[1
]}

Xiao, Haifeng ^{[1
]}

Wei, Huiyan ^{[1
]}

Wang, Wei ^{[1
]}

Zhang, Lei ^{[1
]}

Zhang, Xueying ^{[1
]}

Tang, Qingchao ^{[2
]}

Zhang, Chunjing ^{[4
]}

Tao, Ji ^{[3
]}

Wang, Xishan ^{[2
]}

Gao, Xu ^{[1
,5
]}

机构：

[1] Harbin Med Univ, Dept Biochem & Mol Biol, Harbin, Peoples R China

[2] Harbin Med Univ, Affiliated Hosp 2, Dept Gen Surg, Harbin, Peoples R China

[3] Harbin Med Univ, Affiliated Hosp 3, Dept Med Oncol, Harbin, Peoples R China

[4] Qiqihar Med Univ, Dept Biochem & Mol Biol, Qiqihar, Peoples R China

[5] Minist Educ, Key Lab Cardiovasc Med Res, Harbin, Peoples R China

来源：

CANCER LETTERS | 2013年 / 335卷 / 01期

基金：

中国博士后科学基金;

关键词：

Colon cancer; MicroRNA-25; Smad7; Migration; Proliferation; IDENTIFICATION; EXPRESSION; REGULATOR; CLUSTERS;

D O I：

10.1016/j.canlet.2013.02.029

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Because it is a member of the miR-106b similar to 25 cluster, microRNA-25 (miR-25) is known to be dysregulated in human cancers. However, the expression and role of miR-25 in colon cancer remain unclear. In this study, miR-25 was found to be down-regulated in human colon cancer tissues when compared to those in matched, non-neoplastic mucosa tissues. Functional studies revealed that restoration of miR-25 expression inhibited cell proliferation and migration. In contrast, miR-25 inhibition could promote the proliferation and migratory ability of cells. Stable over-expression of miR-25 also suppressed the growth of colon cancer-cell xenografts in vivo. Furthermore, bioinformatic predictions and experimental validation were used to identify Smad7 as a direct target of miR-25. Functional reverse experiments indicated that the antitumor effects of miR-25 were probably mediated by its repression of Smad7. These results suggest that miR-25 may function as a tumor suppressor by targeting Smad7 in colon cancer. Thus, miR-25 may serve as a potential therapeutic agent or target for cancer therapy. Crown Copyright (c) 2013 Published by Elsevier Ireland Ltd. All rights reserved.

引用

页码：168 / 174

页数：7

共 30 条

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Genomic profiling of MicroRNA and messenger RNA reveals deregulated MicroRNA expression in prostate cancer [J].