Detection of infectious prions in urine

被引：94

作者：

Gonzalez-Romero, Dennisse

Barria, Marcelo A.

Leon, Patricia

Morales, Rodrigo

Soto, Claudio ^{[1
]}

机构：

[1] Univ Texas Med Branch, George & Cynthia Mitchell Ctr Neurodegenerat Dis, Dept Neurol, Galveston, TX 77555 USA

来源：

FEBS LETTERS | 2008年 / 582卷 / 21-22期

关键词：

prion; transmissible spongiform encephalopathy; PMCA; diagnosis; scrapie;

D O I：

10.1016/j.febslet.2008.08.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Prions are the infectious agents responsible for prion diseases, which appear to be composed exclusively by the misfolded prion protein (PrPSc). The mechanism of prion transmission is unknown. In this study, we attempted to detect prions in urine of experimentally infected animals. PrPSc was detected in similar to 80% of the animals studied, whereas no false positives were observed among the control animals. Semi-quantitative calculations suggest that PrPSc concentration in urine is around 10-fold lower than in blood. Interestingly, PrPSc present in urine maintains its infectious properties. Our data indicate that low quantities of infectious prions are excreted in the urine. These findings suggest that urine is a possible source of prion transmission. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：3161 / 3166

页数：6

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