Rapid detection protocol for filoviruses

被引：80

作者：

Weidmann, M

Mühlberger, E

Hufert, FT

机构：

[1] Univ Freiburg, Inst Med Microbiol & Hyg, Dept Virol, D-79104 Freiburg, Germany

[2] Univ Marburg, Inst Virol, D-35037 Marburg, Germany

来源：

JOURNAL OF CLINICAL VIROLOGY | 2004年 / 30卷 / 01期

关键词：

filovirus; Ebola virus; Marburg virus; Taqman; real-time PCR; Lightcycler; Smartcycler; rapid detection;

D O I：

10.1016/j.jcv.2003.09.004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Background: The incidence of filovirus disease outbreaks has been increasing in recent years. Although there have been advances in the developments of diagnostics, field tests are rare. Apart from family members of infected patients, health care workers are at high risk of being infected during the initial phase of an outbreak. RT-PCR has been shown to be helpful in containing outbreaks. Objectives: To develop Taqman-RT-PCR for the detection of Ebola-Zaire virus (EBOV-Z), Ebola-Sudan virus (EBOV-S) and Marburg virus (MBGV). Study desgin: Quantitative Taqman-RT-PCRs for the detection of these viruses were developed and established on a portable Smartcycler TD. Results and conclusions: All three assays were highly sensitive and specific. The mobility of the assay system may help to contain future outbreaks. (C) 2003 Elsevier B.V. All rights reserved.

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页码：94 / 99

页数：6

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