Proliferation and differentiation of rat calvarial osteoblasts on type I collagen-coated titanium alloy

被引:103
作者
Becker, D
Geissler, U
Hempel, U
Bierbaum, S
Scharnweber, D
Worch, H
Wenzel, KW
机构
[1] Dresden Univ Technol, Med Fac Carl Gustav Carus, Inst Physiol Chem, D-01307 Dresden, Germany
[2] Dresden Univ Technol, Inst Sci Mat, Dresden, Germany
来源
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH | 2002年 / 59卷 / 03期
关键词
osteoblast; collagen type I coating; titanium alloy; differentiation; mineralization;
D O I
10.1002/jbm.1265
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Several attempts have been made to improve osseointegration of titanium alloy as an implant material by modification of its surface. In the present study, proliferation, differentiation, and mineralization of osteoblasts on type I collagen-coated Ti6Al4V were investigated. The activity of alkaline phosphatase and the accumulation of calcium by osteoblasts grown on titanium alloy were significantly higher compared to cells grown on polystyrene. Precoating of the implant surface with type I collagen did not extensively affect proliferation, the activity of alkaline phosphatase, collagen synthesis, calcium accumulation, or the mRNA levels for collagen I alpha1, osteopontin, osteocalcin, MMP-2, and TIMP-2. Maximum collagen synthesis by osteoblasts was observed at day 4 of culture independent of the type of implant material. The specific activity of alkaline phosphatase reached its maximum at day 18 of culture. Accumulation of calcium and elevated mRNA levels for osteocalcin were found at day 22. These results indicate that collagen-coating alone is not sufficient to accelerate differentiation of rat calvarial osteoblasts on Ti6Al4V. (C) 2001 John Wiley & Sons, Inc. J Biomed Mater Res 59: 516-527, 2002.
引用
收藏
页码:516 / 527
页数:12
相关论文
共 45 条
[31]   PROGRESSIVE DEVELOPMENT OF THE RAT OSTEOBLAST PHENOTYPE INVITRO - RECIPROCAL RELATIONSHIPS IN EXPRESSION OF GENES ASSOCIATED WITH OSTEOBLAST PROLIFERATION AND DIFFERENTIATION DURING FORMATION OF THE BONE EXTRACELLULAR-MATRIX [J].
OWEN, TA ;
ARONOW, M ;
SHALHOUB, V ;
BARONE, LM ;
WILMING, L ;
TASSINARI, MS ;
KENNEDY, MB ;
POCKWINSE, S ;
LIAN, JB ;
STEIN, GS .
JOURNAL OF CELLULAR PHYSIOLOGY, 1990, 143 (03) :420-430
[32]  
Park JC, 1998, INT J ORAL MAX IMPL, V13, P826
[33]   Investigation of the adsorption of tropocollagen type I on titanium and Ti6Al4V [J].
Rössler, S ;
Scharnweber, D ;
Wolf, C ;
Worch, H .
JOURNAL OF ADHESION SCIENCE AND TECHNOLOGY, 2000, 14 (03) :453-465
[34]   A SEMIAUTOMATED, 96-WELL PLATE ASSAY FOR COLLAGEN-SYNTHESIS [J].
SCUTT, A ;
BERG, A ;
MAYER, H .
ANALYTICAL BIOCHEMISTRY, 1992, 203 (02) :290-294
[35]   High-resolution morphometric analysis of human osteoblastic cell adhesion on clinically relevant orthopedic alloys [J].
Shah, AK ;
Sinha, RK ;
Hickok, NJ ;
Tuan, RS .
BONE, 1999, 24 (05) :499-506
[36]   Mechanism of BMP-2 stimulated adhesion of osteoblastic cells to titanium alloy [J].
Shah, AK ;
Lazatin, J ;
Sinha, RK ;
Lennox, T ;
Hickok, NJ ;
Tuan, RS .
BIOLOGY OF THE CELL, 1999, 91 (02) :131-142
[37]   The role of type I collagen in the regulation of the osteoblast phenotype [J].
Shi, ST ;
Kirk, M ;
Kahn, AJ .
JOURNAL OF BONE AND MINERAL RESEARCH, 1996, 11 (08) :1139-1145
[38]  
Siggelkow H, 1999, J CELL BIOCHEM, V75, P22, DOI 10.1002/(SICI)1097-4644(19991001)75:1<22::AID-JCB3>3.0.CO
[39]  
2-6
[40]   RELATIONSHIP OF CELL-GROWTH TO THE REGULATION OF TISSUE-SPECIFIC GENE-EXPRESSION DURING OSTEOBLAST DIFFERENTIATION [J].
STEIN, GS ;
LIAN, JB ;
OWEN, TA .
FASEB JOURNAL, 1990, 4 (13) :3111-3123