Establishment and application of the yeast two-hybrid (Y2H)-based plant interactome for investigation of gene functions

被引:15
作者
Bang, Bongjun [1 ]
Park, Jungwook [1 ]
Jeon, Jong-Seong [2 ,3 ]
Seo, Young-Su [1 ]
机构
[1] Pusan Natl Univ, Dept Microbiol, Pusan 609735, South Korea
[2] Kyung Hee Univ, Grad Sch Biotechnol, Yongin 446701, South Korea
[3] Kyung Hee Univ, Crop Biotech Inst, Yongin 446701, South Korea
基金
新加坡国家研究基金会;
关键词
Gene function; Network; Plant interactome; Post-genome; Yeast two-hybrid; PROTEIN-PROTEIN INTERACTIONS; FLUORESCENCE COMPLEMENTATION BIFC; TANDEM AFFINITY PURIFICATION; INTERACTION MAP; SUBCELLULAR-LOCALIZATION; NETWORK; KINASES; SYSTEM; COMPLEXES;
D O I
10.1007/s12374-013-0389-7
中图分类号
Q94 [植物学];
学科分类号
071001 [植物学];
摘要
A large amount of genomic information is currently being generated in the biological sciences; however, the postgenome era needs biological connectors to bridge between genome data and gene functions. Interactomes are proteinprotein interaction maps used to study gene function through the demonstration of predicted functions based on known roles of interaction partners to understand biological processes as a systemic approach. Diverse experimental approaches for interactomes have been developed, each of which has its own merits and pitfalls. In this review, the status and application of the yeast two-hybrid (Y2H)-based plant interactome is introduced to investigate gene functions as a biological network.
引用
收藏
页码:367 / 374
页数:8
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