Interaction landscape of membrane-protein complexes in Saccharomyces cerevisiae

被引：179

作者：

Babu, Mohan ^{[2
,3
]}

Vlasblom, James ^{[4
,5
]}

Pu, Shuye ^{[4
]}

Guo, Xinghua ^{[2
]}

Graham, Chris ^{[2
]}

Bean, Bjoern D. M. ^{[1
]}

Burston, Helen E. ^{[1
]}

Vizeacoumar, Franco J. ^{[2
]}

Snider, Jamie ^{[2
]}

Phanse, Sadhna ^{[2
]}

Fong, Vincent ^{[2
]}

Tam, Yuen Yi C. ^{[1
]}

Davey, Michael ^{[1
]}

Hnatshak, Olha ^{[2
]}

Bajaj, Navgeet ^{[2
]}

Chandran, Shamanta ^{[2
]}

Punna, Thanuja ^{[2
]}

Christopolous, Constantine ^{[2
]}

Wong, Victoria ^{[2
]}

Yu, Analyn ^{[2
]}

Zhong, Gouqing ^{[2
]}

Li, Joyce ^{[2
]}

Stagljar, Igor ^{[2
,5
,6
]}

Conibear, Elizabeth ^{[1
]}

Wodak, Shoshana J. ^{[4
,5
,6
]}

Emili, Andrew ^{[2
,6
]}

Greenblatt, Jack F. ^{[2
,6
]}

机构：

[1] Univ British Columbia, Child & Family Res Inst, Ctr Mol Med & Therapeut, Vancouver, BC V5Z 4H4, Canada

[2] Univ Toronto, Donnelly Ctr, Banting & Best Dept Med Res, Toronto, ON M5S 3E1, Canada

[3] Univ Regina, Res & Innovat Ctr, Dept Biochem, Regina, SK S4S 0A2, Canada

[4] Hosp Sick Children, Toronto, ON M5G 1X8, Canada

[5] Univ Toronto, Dept Biochem, Toronto, ON M5S 1A8, Canada

[6] Univ Toronto, Dept Mol Genet, Toronto, ON M5S 1A8, Canada

来源：

NATURE | 2012年 / 489卷 / 7417期

基金：

加拿大健康研究院;

关键词：

PHYSICAL INTERACTOME; ER EXIT; YEAST; MAP; EXPLORATION; SELECTION;

D O I：

10.1038/nature11354

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Macromolecular assemblies involving membrane proteins (MPs) serve vital biological roles and are prime drug targets in a variety of diseases(1). Large-scale affinity purification studies of soluble-protein complexes have been accomplished for diverse model organisms, but no global characterization of MP-complex membership has been described so far. Here we report a complete survey of 1,590 putative integral, peripheral and lipid-anchored MPs from Saccharomyces cerevisiae, which were affinity purified in the presence of non-denaturing detergents. The identities of the co-purifying proteins were determined by tandem mass spectrometry and subsequently used to derive a high-confidence physical interaction map encompassing 1,726 membrane protein-protein interactions and 501 putative heteromeric complexes associated with the various cellular membrane systems. Our analysis reveals unexpected physical associations underlying the membrane biology of eukaryotes and delineates the global topological landscape of the membrane interactome.

引用

页码：585 / +

页数：6

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