A GH3 family member, OsGH3-2, modulates auxin and abscisic acid levels and differentially affects drought and cold tolerance in rice

被引:277
作者
Du, Hao [1 ,2 ]
Wu, Nai [1 ,2 ]
Fu, Jing [1 ,2 ]
Wang, Shiping [1 ,2 ]
Li, Xianghua [1 ,2 ]
Xiao, Jinghua [1 ,2 ]
Xiong, Lizhong [1 ,2 ]
机构
[1] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China
[2] Huazhong Agr Univ, Natl Ctr Plant Gene Res Wuhan, Wuhan 430070, Peoples R China
基金
中国国家自然科学基金;
关键词
ABA; auxin; cold tolerance; drought resistance; GH3; family; Oryza sativa; LATERAL ROOT DEVELOPMENT; 2A REGULATORY SUBUNIT; GENE-EXPRESSION; ABIOTIC STRESS; ARABIDOPSIS-THALIANA; SEED-GERMINATION; RESPONSES; BIOSYNTHESIS; RESISTANCE; ACTIVATION;
D O I
10.1093/jxb/ers300
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Plant responses to abiotic stresses are coordinated by arrays of growth and developmental processes. Indole-3-acetic acid (IAA) and abscisic acid (ABA) play critical roles in developmental programmes and environmental responses, respectively, through complex signalling and metabolism networks. However, crosstalk between the two phytohormones in the stress responses remains largely unknown. Here, it is reported that a GH3 family gene, OsGH3-2, encoding an enzyme catalysing IAA conjugation to amino acids, is involved in the modulation of ABA level and stress tolerance. Expression of OsGH3-2 was induced by drought but was suppressed by cold. Overexpression of OsGH3-2 in rice caused significant morphological aberrations related to IAA deficiency, such as dwarfism, smaller leaves, and fewer crown roots and root hairs. The overexpressing line showed significantly reduced carotene, ABA, and free IAA levels, greater stomata aperture, and faster water loss, and was hypersensitive to drought stress. However, the overexpressing line showed increased cold tolerance, which was due to the combined effects of reduced free IAA content, alleviated oxidative damage, and decreased membrane penetrability. Furthermore, expression levels of some ABA synthesis- and stress-related genes were significantly changed in the overexpression line. It was conclude that OsGH3-2 modulates both endogenous free IAA and ABA homeostasis and differentially affects drought and cold tolerance in rice.
引用
收藏
页码:6467 / 6480
页数:14
相关论文
共 46 条
[31]   Regulatory network of gene expression in the drought and cold stress responses [J].
Shinozaki, K ;
Yamaguchi-Shinozaki, K ;
Seki, M .
CURRENT OPINION IN PLANT BIOLOGY, 2003, 6 (05) :410-417
[32]   Auxin Biosynthesis Inhibitors, Identified by a Genomics-Based Approach, Provide Insights into Auxin Biosynthesis [J].
Soeno, Kazuo ;
Goda, Hideki ;
Ishii, Takahiro ;
Ogura, Takehiko ;
Tachikawa, Tomoe ;
Sasaki, Eriko ;
Yoshida, Shigeo ;
Fujioka, Shozo ;
Asami, Tadao ;
Shimada, Yukihisa .
PLANT AND CELL PHYSIOLOGY, 2010, 51 (04) :524-536
[33]   Characterization of an Arabidopsis enzyme family that conjugates amino acids to indole-3-acetic acid [J].
Staswick, PE ;
Serban, B ;
Rowe, M ;
Tiryaki, I ;
Maldonado, MT ;
Maldonado, MC ;
Suza, W .
PLANT CELL, 2005, 17 (02) :616-627
[34]   Jasmonate response locus JAR1 and several related Arabidopsis genes encode enzymes of the firefly luciferase superfamily that show activity on jasmonic, salicylic, and indole-3-acetic acids in an assay for adenylation [J].
Staswick, PE ;
Tiryaki, I ;
Rowe, ML .
PLANT CELL, 2002, 14 (06) :1405-1415
[35]   FREEZE THAW-INDUCED DESTABILIZATION OF THE PLASMA-MEMBRANE AND THE EFFECTS OF COLD-ACCLIMATION [J].
STEPONKUS, PL ;
LYNCH, DV .
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 1989, 21 (01) :21-41
[36]   Maize VP1 complements Arabidopsis abi3 and confers a novel ABA/auxin interaction in roots [J].
Suzuki, M ;
Kao, CY ;
Cocciolone, S ;
McCarty, DR .
PLANT JOURNAL, 2001, 28 (04) :409-418
[37]   DROUGHT RHIZOGENESIS IN ARABIDOPSIS-THALIANA - DIFFERENTIAL RESPONSES OF HORMONAL MUTANTS [J].
VARTANIAN, N ;
MARCOTTE, L ;
GIRAUDAT, J .
PLANT PHYSIOLOGY, 1994, 104 (02) :761-767
[38]   Methods and concepts in quantifying resistance to drought, salt and freezing, abiotic stresses that affect plant water status [J].
Verslues, PE ;
Agarwal, M ;
Katiyar-Agarwal, S ;
Zhu, JH ;
Zhu, JK .
PLANT JOURNAL, 2006, 45 (04) :523-539
[39]   A dynamic gene expression atlas covering the entire life cycle of rice [J].
Wang, Lei ;
Xie, Weibo ;
Chen, Ying ;
Tang, Weijiang ;
Yang, Jiangyi ;
Ye, Rongjian ;
Liu, Li ;
Lin, Yongjun ;
Xu, Caiguo ;
Xiao, Jinghua ;
Zhang, Qifa .
PLANT JOURNAL, 2010, 61 (05) :752-766
[40]   Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses [J].
Yamaguchi-Shinozaki, Kazuko ;
Shinozaki, Kazuo .
ANNUAL REVIEW OF PLANT BIOLOGY, 2006, 57 :781-803