Chondrogenic potential of IL-10 in mechanically injured cartilage and cellularized collagen ACI grafts

被引：61

作者：

Behrendt, P. ^{[1
,2
]}

Feldheim, M. ^{[3
]}

Preusse-Prange, A. ^{[3
]}

Weitkamp, J. T. ^{[3
]}

Haake, M. ^{[3
]}

Eglin, D. ^{[2
]}

Rolauffs, B. ^{[4
]}

Fay, J. ^{[5
]}

Seekamp, A. ^{[1
]}

Grodzinsky, A. J. ^{[6
,7
,8
]}

Kurz, B. ^{[3
]}

机构：

[1] Univ Med Ctr Schleswig Holstein, Dept Orthoped & Trauma Surg, Campus Kiel, Kiel, Germany

[2] AO Res Inst, Davos, Switzerland

[3] Univ Kiel, Inst Anat, Kiel, Germany

[4] Albert Ludwigs Univ Freiburg, GERN Tissue Replacement Regenerat & Neogenesis, Dept Orthoped & Trauma Surg, Med Ctr,Fac Med, Freiburg, Germany

[5] Lubinus Clin, Dept Trauma & Sports Orthoped, Kiel, Germany

[6] MIT, Dept Biol Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[7] MIT, Dept Elect Engn & Comp Sci, Cambridge, MA 02139 USA

[8] MIT, Dept Mech Engn, Cambridge, MA 02139 USA

来源：

OSTEOARTHRITIS AND CARTILAGE | 2018年 / 26卷 / 02期

关键词：

Cartilage; IL-10; Injurious compression; Osteoarthritis; Autologous chondrocyte implantation; HUMAN ARTICULAR CHONDROCYTES; GENE-EXPRESSION; TNF-ALPHA; IN-VITRO; INJURIOUS COMPRESSION; MONOLAYER-CULTURE; SYNOVIAL-FLUID; II COLLAGEN; KNEE; INTERLEUKIN-10;

D O I：

10.1016/j.joca.2017.11.007

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

100224 [整形外科学];

摘要：

Objective: The application of adjunctive mediators in Autologous chondrocyte implantation (ACI) techniques might be useful for improving the dedifferentiated chondrocyte phenotype, to support neocartilage formation and inhibit post-traumatic cartilage destruction. In this study we examined if (a) interleukin 10 treatment can cause chondrogenic phenotype stabilization and matrix preservation in mechanically injured cartilage and if (b) IL-10 can promote chondrogenesis in a clinically applied collagen scaffold for ACI treatment. Materials and methods: For (a) bovine articular cartilage was harvested, subjected to an axial unconfined injury and treated with bovine IL-10 (1-10,000 pg/ng/ml). For (b) a post-operatively remaining ACI graft was treated with human IL-10. Expression levels of type I/II/X collagen, SOX9 and aggrecan were measured by qPCR (a, b). After 3 weeks cell death was analyzed (nuclear blebbing and TUNEL assay) and matrix composition was determined by GAG measurements and immunohistochemistry (aggrecan, type I/II collagen, hyaluronic acid). Statistics: One way ANOVA analysis with Bonferroni's correction. Results: (a) IL-10 stabilized the chondrogenic phenotype after injurious compression and preserved matrix integrity. This was indicated by elevated expression of chondrogenic markers COL2A1, ACAN, SOX9, while COL1A1 and COL10A1 were reduced. An increased GAG content paralleled this and histological staining of type 2 collagen, aggrecan and toluidine blue were enhanced after 3 weeks. (b) IL-10 [100 pg/ml] improved the chondrogenic differentiation of human chondrocytes, which was accompanied by cartilaginous matrix formation after 3 weeks of incubation. Conclusion: Interleukin-10 is a versatile adjuvant candidate to control the post-injurious environment in cartilage defects and promote chondrogenesis in ACI grafts. (C) 2017 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

引用

页码：264 / 275

页数：12

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